Diagnostic Significance of miR-937 in Peripheral Blood Mononuclear Cells of Kawasaki Disease
Diagnostic Significance of miR-937 in Peripheral Blood Mononuclear Cells of Kawasaki Disease
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DOI：
10.7754/clin.lab.2019.190507
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发表时间：
2019-01-01
影响因子：
0.7
通讯作者：
Li, Wei
中科院分区：
文献类型：
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作者：
Wang, Zhenyu;Zhou, Jing;Li, Wei
本研究旨在探讨miR-937能否作为川崎病(KD)伴或不伴冠状动脉扩张(CAD)患儿外周血单个核细胞(PBMC)的生物标志物。采用实时荧光定量聚合酶链式反应检测50例KD患儿(冠心病组25例，非冠心病组25例)和25例健康儿童的miR-937基因的表达。结果：首先，利用基因芯片技术鉴定出20个KD患儿与正常儿童外周血单个核细胞(PBMC)差异表达的miR。实时荧光定量PCR分析证实，miR-937在所有差异表达的miRNAs中表达下降最为显著。丙种球蛋白(IVIG)治疗前miR-937表达显著下调，IVIG治疗后miR-937表达显著上调。另外，KD伴CAD组miR-937的表达明显低于KD非CAD组。个人相关分析显示miR-937与冠心病呈负相关。双荧光素酶报告基因分析表明IL-1β是miR-937的靶基因。结论：PBMCs中的miR-937参与了KD的发生发展，为防治KD冠状动脉扩张提供了新的思路。
Beckground: The current study aims to investigate whether miR-937 can be used as a diagnostic biomarker in peripheral blood mononuclear cells (PBMCs) for children with Kawasaki disease (KD) with or without coronary artery dilation (CAD).Methods: Gene chip technology was used to screen miRNAs differentially expressed between KD children and normal healthy children. Furthermore, real time PCR was carried out to validate the expression of miR-937 in 50 children with KD (25 cases with and 25 cases without CAD) and 25 healthy children. Meanwhile, target genes of miR-937 were analyzed using TargetScan and dual luciferase reporter assay.Results: First, 20 miRs with significantly differentially expressed mononuclear cell (PBMCs) in peripheral blood between children with KD and normal healthy children were identified by gene chip technology. Real time PCR analysis validated that the expression of miR-937 decreased most significantly among all differentially expressed miRNAs. Secondly, miR-937 was down-regulated significantly before treatment with gamma globulin (IVIG), while its expression was significantly up-regulated after IVIG treatment. In addition, the expression of miR-937 in KD children with CAD was significantly lower than that of KD children without CAD. Person's correlation assay showed that miR-937 negatively correlated with CAD. Dual luciferase reporter assay indicated that IL-1 beta was a target gene of miR-937.Conclusions: In summary, miR-937 in PBMCs was involved in the occurrence and development of KD, which provides new ideas for the prevention and treatment of KD coronary artery dilation.