Identification of piRNAs in Hela cells by massive parallel sequencing
Identification of piRNAs in Hela cells by massive parallel sequencing
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通过大规模并行测序鉴定 Hela 细胞中的 piRNA。
DOI：
10.3858/bmbrep.2010.43.9.635
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发表时间：
2010-09-30
期刊：
影响因子：
3.8
通讯作者：
Ma, Yongxin
中科院分区：
文献类型：
--
作者：
Lu, Yilu;Li, Chao;Ma, Yongxin
Piwi蛋白和Piwi相互作用RNA（piRNA）已经涉及从果蝇到哺乳动物的生殖系中的转座子控制。为了检查人癌细胞中小RNA表达谱并探索小RNA转录组的差异，使用Solexa技术对从野生型、HILI过表达和HILI敲减的Hela细胞制备的小RNA文库进行测序。在Hela细胞中观察到piRNA和其他重复相关的小RNA。原位杂交结果表明，piR-49322定位于Hela细胞的核仁和核膜周围。在HILI过表达后，逆转录转座子元件LINE 1被显著抑制，而LINE 1相关的小RNA丰度减少。本研究证明HILI沿着piRNA在Hela癌细胞系中的LINE 1抑制中起作用。[BMB报告2010; 43（9）：635-641]
Piwi proteins and Piwi-interacting RNAs (piRNAs) have been implicated in transposon control in germline from Drosophila to mammals. To examine the profile of small RNA expression in human cancer cells and explore difference in small RNA transcriptome, small RNA libraries prepared from wildtype, HILI overexpressed and HILI knockdowned Hela cells were sequenced using Solexa technology. piRNAs and other repeat-associated small RNAs were observed in Hela cells. By using in situ hybridization, piR-49322 was localized in the nucleolus and around the periphery of nuclear membrane in Hela cells. Following the overexpression of HILI, the retro-transposon elements LINE1 was significantly repressed, while LINE1-associated small RNAs decreased in abundance. The present study demonstrated that HILI along with piRNAs plays a role in LINE1 suppression in Hela cancer cell line. [BMB reports 2010; 43(9): 635-641]