Novel Glycosaminoglycan Glycotechnology: Method for Hybrid Synthesis of Glycosaminoglycan Chains Utilizing Chemo-enzymatic Procedures
Novel Glycosaminoglycan Glycotechnology: Method for Hybrid Synthesis of Glycosaminoglycan Chains Utilizing Chemo-enzymatic Procedures
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DOI：
10.1080/07328303.2010.514488
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发表时间：
2010-01-01
影响因子：
1
通讯作者：
Endo, Masahiko
中科院分区：
文献类型：
--
作者：
Yamaguchi, Masanori;Kakizaki, Ikuko;Endo, Masahiko
描述了一种合成糖胺聚糖链的新方法。糖胺聚糖经消除酶消化后，其非还原末端的4，5-不饱和己糖醛酸通过氧汞化-脱汞反应转化为葡萄糖醛酸。然后，利用这些寡糖作为受体的牛睾丸透明质酸酶的转糖基化反应，糖胺聚糖寡糖被成功地构建。认为本方法将有助于糖胺聚糖糖生物学和技术的发展。
A novel method for the synthesis of glycosaminoglycan chains is described. The 4,5-unsaturated hexuronic acid located at the nonreducing terminal, which derived from eliminase digestion of glycosaminoglycan, is chemically converted to glucuronic acid by employing the oxymercuration-demercuration reaction. Then, utilizing these oligosaccharides as acceptors for the transglycosylation reaction of bovine testicular hyaluronidase, glycosaminoglycan oligosaccharides were successfully constructed. It is thought that the present method will contribute to the development of glycosaminoglycan glycobiology and technology.