TRANSFORMING GROWTH-FACTOR-BETA - DOES IT DIRECT OTIC CAPSULE FORMATION
TRANSFORMING GROWTH-FACTOR-BETA - DOES IT DIRECT OTIC CAPSULE FORMATION
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DOI：
10.1177/000348949110000407
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发表时间：
1991-04-01
影响因子：
1.4
通讯作者：
GALINOVICSCHWARTZ, V
中科院分区：
文献类型：
--
作者：
FRENZ, DA;VANDEWATER, TR;GALINOVICSCHWARTZ, V
耳囊肿上皮与周围的周间质之间的相互作用指导哺乳动物内耳被膜的形成。在本研究中，我们对胚胎发育10至14天内耳上皮和间充质组织中转化生长因子β 1 （tgf - β 1）的时空分布进行了表征。此外，利用高密度培养的骨膜间充质来模拟软骨囊形成，我们已经证明外源性tgf - β -1可以通过增强或抑制这一过程来调节软骨形成。我们的免疫组织化学和体外结果表明，这种生长因子在调节上皮-间充质组织相互作用和形态发生方面具有旁分泌作用。
Interactions between the epithelium of the otocyst and surrounding periotic mesenchyme direct the formation of the capsule of the mammalian inner ear. In the present study, we have characterized the temporal-spatial distribution of transforming growth factor beta 1 (TGF-beta-1) in the epithelial and mesenchymal tissues that compose the inner ear between 10 and 14 days of embryonic development. In addition, using high-density cultures of periotic mesenchyme to model otic capsule formation, we have demonstrated that exogenous TGF-beta-1 can modulate otic chondrogenesis by acting as either an enhancer or a suppressor of this process. Our immunohistochemical and in vitro results suggest a paracrine action for this growth factor in modulation of epithelial-mesenchymal tissue interactions and otic morphogenesis.