GSH-ZnS Nanoparticles Exhibit High-Efficiency and Broad-Spectrum Antiviral Activities via Multistep Inhibition Mechanisms
GSH-ZnS Nanoparticles Exhibit High-Efficiency and Broad-Spectrum Antiviral Activities via Multistep Inhibition Mechanisms
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GSH-ZnS 纳米颗粒通过多步抑制机制表现出高效、广谱的抗病毒活性
DOI：
10.1021/acsabm.0c00332
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发表时间：
2020
影响因子：
4.7
通讯作者：
Shaobo Xiao
中科院分区：
文献类型：
--
作者：
Yanrong Zhou;Ting Tong;Xiaohan Jiang;Liurong Fang;Yuan Wu;Jiangong Liang;Shaobo Xiao
尽管硫化锌纳米颗粒具有良好的生物相容性和抗菌活性，但其是否具有抗病毒活性仍不清楚。本文合成了GSH修饰的ZnS纳米粒（GSH-ZnS NPs），并以动脉炎病毒科RNA病毒、猪繁殖与呼吸综合征病毒（PRRSV）为模型，证明了其显著的抗病毒活性。从机制上讲，GSH-ZnS NP显示出减少PRRSV诱导的ROS产生以防止PRRSV增殖，而对干扰素（IFN）信号通路（对抗病毒感染的第一道防线）没有激活作用。此外，同量异序标签的相对和绝对定量（iTRAQ）为基础的定量蛋白质组学分析的GSH-ZnS NP处理的细胞揭示了参与病毒增殖的许多关键蛋白质，玻连蛋白（VTN）被确认为一个有效的PRRSV拮抗剂在这里。此外，GSH-ZnS纳米粒对疱疹病毒科DNA病毒、伪狂犬病病毒（PRV）、冠状病毒科正义RNA病毒、猪流行性腹泻病毒（PEDV）和弹状病毒科负链RNA病毒、水泡性口炎病毒（VSV）均具有较强的抗病毒活性，表明GSH-ZnS纳米粒具有广谱抗病毒活性。总的来说，GSH-ZnS NP是抗病毒纳米材料开发的一个有前景的候选者，并可作为与蛋白质组学相结合的潜在宿主限制因素的研究模型。
Despite the good biocompatibility and antibacterial activity of zinc sulfide nanoparticles (ZnS NPs), whether they possess antiviral activity is still unclear. Here, GSH-modified ZnS NPs (GSH-ZnS NPs) were synthesized and their significant antiviral activity was demonstrated using theArteriviridaefamily RNA virus, porcine reproductive and respiratory syndrome virus (PRRSV), as a model. Mechanistically, GSH-ZnS NPs were shown to reduce PRRSV-induced ROS production to prevent PRRSV multiplication, with no activating effect on the interferon (IFN) signal pathway, the first defense line against virus infection. Furthermore, isobaric tags for relative and absolute quantification (iTRAQ)-based quantitative proteomic analysis of GSH-ZnS NP-treated cells revealed the involvement of numerous crucial proteins in virus proliferation, with vitronectin (VTN) being confirmed as an efficient PRRSV antagonist here. Furthermore, GSH-ZnS NPs were found to have potent antiviral effects on theHerpesviridaefamily DNA virus, pseudorabies virus (PRV), theCoronaviridaefamily positive-sense RNA virus, porcine epidemic diarrhea virus (PEDV), and theRhabdoviridaefamily negative-stranded RNA virus, vesicular stomatitis virus (VSV), indicating their broad-spectrum antiviral activity against viruses from different families with various genome types. Overall, GSH-ZnS NP is a prospective candidate for the development of antiviral nanomaterials and may serve as a model for investigation of potential host restriction factors in combination with proteomics.