ECTOPIC EXPRESSION OF THE PROTO-ONCOGENE INT-1 IN XENOPUS EMBRYOS LEADS TO DUPLICATION OF THE EMBRYONIC AXIS
ECTOPIC EXPRESSION OF THE PROTO-ONCOGENE INT-1 IN XENOPUS EMBRYOS LEADS TO DUPLICATION OF THE EMBRYONIC AXIS
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DOI：
10.1016/0092-8674(89)90506-0
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发表时间：
1989-09-22
期刊：
影响因子：
64.5
通讯作者：
MOON, RT
中科院分区：
文献类型：
--
作者：
MCMAHON, AP;MOON, RT
虽然有令人信服的证据表明果蝇int-1参与了模式调控，但int-1在脊椎动物发育中的正常作用尚不清楚。我们已经将小鼠int-1 RNA注射到非洲爪哇卵中，并监测了后续的发育情况。注射的RNA被翻译，蛋白质广泛分布。胚胎发育成明显正常的原肠，但几乎所有存活的神经细胞都有分叉的前神经板和扩张的后神经板。神经板的分叉可以通过替换一个保守的半胱氨酸残基来消除，并依赖于INT-1蛋白中信号肽序列的存在。组织学检查表明，其下的中轴中胚层结构是重复的。这一结果表明，异位INT-1的表达导致了双轴的形成，并表明INT-1在脊椎动物发育的模式形成过程中发挥了作用。
While there is convincing evidence implicating Drosophila int-1 in pattern regulation, the normal role of int-1 in vertebrate development is unclear. We have injected Xenopus eggs with mouse int-1 RNA and monitored subsequent development. Injected RNA is translated and the protein widely distributed. Embryos develop into apparently normal gastrulae, but almost all surviving neurulae have a bifurcated anterior and expanded posterior neural plate. Bifurcation of the neural plate was abolished by substitution of a single, conserved cysteine residue and was dependent on the presence of a signal peptide sequence in the int-1 protein. Histological examination indicates that underlying axial mesodermal structures were duplicated. This result suggests that ectopic int-1 expression leads to dual axis formation and points to a role for int-1 in patterning processes in vertebrate development.