Immunohistochemical analysis of CCN2 in experimental fracture healing models
Immunohistochemical analysis of CCN2 in experimental fracture healing models
复制标题
实验性骨折愈合模型中 CCN2 的免疫组织化学分析
DOI：
10.1007/978-1-0716-2744-0_23
复制
发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Akihiro Hosoya
中科院分区：
文献类型：
--
作者：
Tsuyoshi Shimo;Hiroaki Takebe;Saki Fujii;Akihiro Hosoya
颅骨骨折是最常见的大器官创伤性损伤，影响功能和美学结果以及生活质量。不幸的是，骨折愈合过程中的感染和骨的血液供应不足阻碍了产生软骨和有效骨痂形成的能力降低，导致骨折不愈合或延迟愈合。因此，研究骨折愈合机制是解决骨折愈合失败问题的重要课题。骨折愈合的动物模型是研究其发病机制和制定治疗策略的重要工具。该方案向研究人员介绍了利用大鼠肋骨的骨修复模型和骨折愈合过程中细胞通讯网络因子/结缔组织生长因子（CTGF/CCN 2）的免疫组织学表达。
Skeletal fractures are most common large-organ traumatic injuries that impact the functions and esthetic outcomes and quality of life. Unfortunately, infection during the fracture healing process and inadequate blood supply to the bone impede reduced ability to produce cartilage and effective bone callus formation, leading to nonunion or delayed union fracture. Therefore, studying the mechanism of fracture healing is an important task in solving the problem of fracture healing failure. Animal models of bone fracture healing are important tools to investigate the pathogenesis and develop treatment strategies. This protocol introduces researchers to a bone repair model utilizing the ribs of rats and the immunohistological expression of cellular communication network factor/connective tissue growth factor (CTGF/CCN2) during the fracture healing processes.