Ontogeny of substance P-containing neurons in relation to serotonin-containing neurons in the central nervous system of the mouse.
Ontogeny of substance P-containing neurons in relation to serotonin-containing neurons in the central nervous system of the mouse.
复制标题
小鼠中枢神经系统中含有 P 物质的神经元与含有血清素的神经元的个体发育。
DOI：
10.1016/0306-4522(89)90371-0
复制
发表时间：
1989
期刊：
影响因子：
3.3
通讯作者：
Jonakait,GM
中科院分区：
文献类型：
--
作者：
Ni,L;Jonakait,GM
In order to begin to investigate the developmental factors influencing co-localized neurotransmitters in the central nervous system, we have studied the ontogenesis of coexisting transmitters, substance P and serotonin, in cells of the medullary raphe of the fetal mouse using double-label immunocytochemistry. The results indicate that initial detection of these neurotransmitters occurs at embryonic day 12 in non-overlapping cell populations. Substance P-like immunoreactivity is first co-localized with serotonin in cells of the caudal medullary raphe (raphe pallidus and raphe obscurus) at embryonic day 13. The percentage of serotonin cells containing substance P was estimated at embryonic day 13 and subsequent gestational ages both by double-label immunofluorescence using fluorescein- and rhodamine-conjugated secondary antibodies and also by the elution technique. Double-label immunofluorescence routinely and reliably yielded the highest proportion of co-localized cells. At embryonic day 13, 89% of serotonin cells contained substance P-like immunoreactivity. A consistently high degree of co-localization occurred throughout embryonic development (87% at postnatal day 3) and into adulthood (87%). Colchicine treatment was required at older ages to elicit these data.Unexpectedly, we found that neurons containing substance P-like immunoreactivity in the piriform cortex and in the hypothalamus transiently expressed serotonin immunoreactivity during normal ontogenesis.
