An investigation into the effect of surface roughness of stainless steel on human umbilical vein endothelial cell gene expression
An investigation into the effect of surface roughness of stainless steel on human umbilical vein endothelial cell gene expression
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DOI：
10.1080/10623320600660185
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发表时间：
2006-01-01
影响因子：
--
通讯作者：
Smith, TJ
中科院分区：
文献类型：
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作者：
McLucas, E;Moran, MT;Smith, TJ
血管器械的表面特性决定了发生的初始植入后反应，从而决定了植入手术的有效性。在过去的几年中，已经出现了许多不同的支架设计，并且支架通常在制造过程中被抛光至镜面光洁度。本研究旨在使用适当的细胞培养体外测定系统研究不锈钢表面粗糙度对内皮细胞基因表达的影响。不锈钢圆盘通过喷丸处理粗糙化或通过机械抛光抛光。通过原子力显微镜（AFM）测定经处理和未经处理的盘的表面粗糙度。将细胞接种在1型胶原蛋白凝胶上并使其附着24小时。然后将不同粗糙度的不锈钢盘与细胞接触并孵育24小时。然后进行RNA提取和定量实时逆转录酶-聚合酶链反应（RT-PCR），以确定与合适的对照细胞相比，经处理的细胞中候选基因的表达水平。发现E-选择素和血管细胞粘附分子（VCAM-1）在与抛光和粗糙样品孵育的细胞中显著上调，表明内皮细胞活化和炎症。本研究表明，不锈钢的表面粗糙度是血管支架开发中的重要表面性能。
The surface properties of vascular devices dictate the initial postimplantation reactions that occur and thus the efficacy of the implantation procedure. Over the last number of years, a number of different stent designs have emerged and stents are generally polished to a mirror finish during the manufacturing procedure. This study sought to investigate the effect of stainless steel surface roughness on endothelial cell gene expression using an appropriate cell culture in vitro assay system. Stainless steel discs were roughened by shot blasting or polished by mechanical polishing. The surface roughness of the treated and untreated discs was determined by atomic force microscopy (AFM). Cells were seeded on collagen type 1 gels and left to attach for 24 h. Stainless steel discs of varying roughness were then placed in contact with the cells and incubated for 24 h. RNA extractions and quantitative real-time reverse transcriptase-polymerase chain reaction (RT-PCR) was then performed to determine the expression levels of candidate genes in the treated cells compared to suitable control cells. E-selectin and vascular cellular adhesion molecule (VCAM-1) were found to be significantly up-regulated in cells incubated with polished and roughened samples, indicating endothelial cell activation and inflammation. This study indicates that the surface roughness of stainless steel is an important surface property in the development of vascular stents.