Serum miR-21 and miR-92a as biomarkers in the diagnosis and prognosis of colorectal cancer
Serum miR-21 and miR-92a as biomarkers in the diagnosis and prognosis of colorectal cancer
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DOI：
10.1007/s13277-013-0753-8
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发表时间：
2013-08-01
期刊：
影响因子：
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通讯作者：
Sun, Xiao-Feng
中科院分区：
文献类型：
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作者：
Liu, Guang-Hui;Zhou, Zong-Guang;Sun, Xiao-Feng
我们实验室先前的研究鉴定了许多在结直肠癌（CRC）组织中异常表达的miRNA。然而，它们在血清中的诊断和预后价值尚未得到充分评价。在本研究中，我们通过实时定量聚合酶链反应（RT-PCR）检测了200例CRC患者、50例晚期腺瘤患者和80例健康对照血清中5种miRNA（miR-21、miR-31、miR-92 a、miR-18 a和miR-106 a）的水平。在我们的研究中，CRC和进展期腺瘤患者的miR-21和miR-92 a水平显著高于健康对照组（均P <0. 05）。在区分CRC与对照中，miR-21产生0.802的受试者工作特征（ROC）曲线下面积（AUC），miR-92 a产生0.786的AUC。此外，miR-21和miR-92 a在区分晚期腺瘤与对照中的AUC分别为0.709和0.701。使用两种miRNA的组合ROC分析揭示了在区分CRC中升高的0.847的AUC，以及在区分晚期腺瘤与对照中升高的0.722的AUC。在多变量考克斯比例风险分析中，CRC中miR-92 a的高表达与生存率差独立相关（P = 0.03;风险比4.36; 95%置信区间= 1.64-11.57）。在CRC、进展期腺瘤和对照样本中未观察到miR-18 a、miR-31和miR-106 a的水平存在显著差异。总之，我们的数据表明，miR-21和miR-92 a血清水平对CRC的早期检测具有潜在价值。此外，miR-92 a在CRC患者中具有预后价值。
Previous studies from our laboratory identified a number of miRNAs that were aberrantly expressed in colorectal cancer (CRC) tissue. However, their diagnostic and prognostic value in serum has not been fully evaluated. In the present study, we measured the levels of five miRNAs (miR-21, miR-31, miR-92a, miR-18a, and miR-106a) in serum samples from 200 CRC patients, 50 advanced adenoma patients, and 80 healthy controls by real-time quantitative polymerase chain reaction (RT-PCR). In our study, the levels of miR-21 and miR-92a in patients with CRC and advanced adenoma were significantly higher than those in healthy controls (all P < 0.05). MiR-21 yielded an area under the receiver-operating characteristics (ROC) curve (AUC) of 0.802 and miR-92a yielded an AUC of 0.786 in discriminating CRCs from the controls. Additionally, miR-21 and miR-92a yielded an AUC of 0.709 and 0.701, respectively, in discriminating advanced adenomas from the controls. Combined ROC analyses using both miRNAs, revealed an elevated AUC of 0.847 in discriminating CRCs, and an AUC of 0.722 in discriminating advanced adenomas from the controls. In the multivariate Cox proportional hazards analysis, high miR-92a expression in CRC was independently associated with poor survival (P = 0.03; hazard ratio 4.36; 95 % confidence interval = 1.64-11.57). No significant difference was observed in the levels of miR-18a, miR-31, and miR-106a among CRC, advanced adenoma, and control samples. In summary, our data indicate that miR-21 and miR-92a serum levels have potential value for early detection of CRC. Furthermore, miR-92a has prognostic value in CRC patients.