Anandamide and diet:: Inclusion of dietary arachidonate and docosahexaenoate leads to increased brain levels of the corresponding N-acylethanolamines in piglets
Anandamide and diet:: Inclusion of dietary arachidonate and docosahexaenoate leads to increased brain levels of the corresponding N-acylethanolamines in piglets
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DOI：
10.1073/pnas.101119098
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发表时间：
2001-05-22
影响因子：
11.1
通讯作者：
Di Marzo, V
中科院分区：
文献类型：
--
作者：
Berger, A;Crozier, G;Di Marzo, V
大麻素受体的内源性配体最近已被发现，并且包括一些N-酰基乙醇胺（NAE;例如，N-Ayachidonoyethanolamine）和一些2-酰基甘油（例如，Sn-2-花生四烯酰甘油）。以前，我们发现这些化合物是生物活性时，给药口服大量的小鼠。在本研究中，在仔猪出生后的前18天内，以与猪乳汁中发现的水平相似的水平，饲喂含和不含NAE的20：4 n-6和22：6 n-3脂肪酸前体的饲料，并评估相应的脑NAE。在饲喂含20：4 n-6和22：6 n-3饲料的仔猪中，脑匀浆中的几种生物活性NAE增加-20：4 n-6 NAE（4倍）、20：5 n-3 NAE（5倍）以及22：5 n-3和22：6 n-3 NAE（9至10倍）。这些结果支持了我们提出的膳食长链多不饱和脂肪酸对脑生化影响的机制，并推测其可能具有功能后遗症。这种模式将使有针对性的调查，以确定是否和为什么特定人群，如婴儿，老年人，或患有某些临床疾病的人可能受益于膳食长链多不饱和脂肪酸。
Endogenous ligands of cannabinoid receptors have been discovered recently and include some N-acylethanolamines (NAEs; e,g., N-ayachidonoylethanolamine) and some 2-acylglycerols (e.g., sn-2-arachidonoylglycerol). Previously, we found these compounds to be active biologically when administered per os in large quantities to mice. In the present work, piglets were fed diets with and without 20:4n-6 and 22:6n-3 fatty acid precursors of NAEs, in levels similar to those found in porcine milk, during the first 18 days of life, and corresponding brain NAEs were assessed. In piglets fed diets containing 20:4n-6 and 22:6n-3, there were increases in several biologically active NAEs in brain homogenates-20:4n-6 NAE (4-fold), 20:5n-3 NAE (5-fold), and 22:5n-3 and 22:6n-3 NAE (9- to 10-fold). These results support a mechanism we propose for dietary long-chain polyunsaturated fatty acids influences on brain biochemistry with presumed functional sequelae. This paradigm will enable targeted investigations to determine whether and why specific populations such as infants, elderly, or persons suffering from certain clinical conditions may benefit from dietary long-chain polyunsaturated fatty acids.