Investigation of Hyperpolarization-activated Cyclic Nucleotide-gated Channels in Interstitial Cells of Cajal of Human Bladder
Investigation of Hyperpolarization-activated Cyclic Nucleotide-gated Channels in Interstitial Cells of Cajal of Human Bladder
复制标题
人膀胱Cajal间质细胞超极化激活的环核苷酸门控通道的研究
DOI：
10.1016/j.urology.2012.04.005
复制
发表时间：
2012-07-01
期刊：
影响因子：
2.1
通讯作者：
Wang, He
中科院分区：
文献类型：
--
作者：
Xue, Li;Li, Yuxin;Wang, He
目的研究超极化活化环核苷酸门控蛋白（hyperpolarization-activated cyclic nucleotide-gated， HCN）在人膀胱Cajal间质细胞（ICCs）中的表达，并确定HCN亚型的相对表达量。方法采集膀胱标本30例，每个膀胱标本分为2部分。第一部分包括粘膜、粘膜下层和肌肉，用于免疫荧光研究。第二部分，切除粘膜和粘膜下层，保留新鲜膀胱肌肉组织，进行Western blotting。首先，利用免疫荧光技术检测HCN1、HCN2、HCN3和HCN4亚型在固有层ICCs中的免疫反应性。其次，采用Western blotting分析膀胱逼尿肌icc中HCN1、HCN2、HCN3和HCN4亚型的蛋白表达。结果免疫荧光显示，在固有层区和逼尿肌有大量vimentin和hcn4阳性ICCs。此外，仅在固有层区和逼尿肌中检测到少量c-kit和hcn4阳性icc。此外，在固有层中发现新的c-kit阴性，但hcn4阳性的肥大细胞。免疫荧光和Western blot结果显示，HCN1、HCN2、HCN3、HCN4通道均存在于膀胱ICCs中；然而，HCN4通道在icc中的表达高于HCN1、HCN2和HCN3。结论HCN1、HCN2、HCN3、HCN4通道在人膀胱组织icc中均有表达，且HCN4通道的表达量高于其他亚型。泌尿外科杂志，21(4):344 - 344。e18, 2012年。(C) 2012爱思唯尔公司
OBJECTIVE To investigate the expression of hyperpolarization-activated cyclic nucleotide-gated (HCN) protein in the interstitial cells of Cajal (ICCs) of the human bladder and to determine the relative expression of the HCN subtypes.METHODS A total of 30 bladder specimens were obtained, and each bladder sample was divided into 2 parts. Part I, which included mucosa, submucosa, and muscle, was used for immunofluorescence study. Part II, in which the mucosa and submucosa were removed and fresh bladder muscle tissue was kept, was used for Western blotting. First, the immunoreactivity of HCN1, HCN2, HCN3, and HCN4 isoforms was detected in the ICCs of lamina propria using immunofluorescence. Second, the protein expression of HCN1, HCN2, HCN3, and HCN4 isoforms in ICCs of bladder detrusor muscle was analyzed using Western blotting.RESULTS Immunofluorescence showed that there were many vimentin- and HCN4-positive ICCs in the lamina propria region and detrusor. Also, only a few c-kit and HCN4-positive ICCs were detected in the lamina propria region and detrusor. Additionally, novel c-kit negative, but HCN4-positive mast cells were found in the lamina propria. Immunofluorescence and Western blotting revealed that HCN1, HCN2, HCN3, and HCN4 channels were all present in the ICCs of the human bladder; however, HCN4 channel expression in the ICCs was greater than in HCN1, HCN2, or HCN3.CONCLUSION HCN1, HCN2, HCN3, and HCN4 channels are identified in ICCs of human bladder tissue, and the expression of the HCN4 channel exceeded that of the other subtypes. UROLOGY 80: 224.e13-224.e18, 2012. (C) 2012 Elsevier Inc.