MOLECULAR CHARACTERIZATION, EXPRESSION AND LOCALIZATION OF HUMAN NEUROKININ-3 RECEPTOR
MOLECULAR CHARACTERIZATION, EXPRESSION AND LOCALIZATION OF HUMAN NEUROKININ-3 RECEPTOR
复制标题
DOI：
10.1016/0014-5793(92)80107-r
复制
发表时间：
1992-03-02
期刊：
影响因子：
3.5
通讯作者：
KAWASHIMA, E
中科院分区：
文献类型：
--
作者：
BUELL, G;SCHULZ, MF;KAWASHIMA, E
通过对人基因组片段的DNA序列分析，推导出人神经激肽-3受体的完整氨基酸序列。将预测的一级结构与人神经激肽受体1和2的一级结构进行比较，发现七个疏水区高度保守，其中最大的分歧出现在氨基和羧基末端。内含子-外显子连接的位置与其他已报道的神经激肽基因的位置相同。利用嵌合基因组-cDNA基因，人NK-3受体在非洲爪哇卵母细胞中表达，它介导膜电导对其激动剂神经激肽B的响应。更重要的是，该基因在哺乳动物细胞中的表达导致了受体结合的检测以及神经激肽刺激的钙动员和花生四烯酸释放，所有这些都表现出神经激肽-3受体的药理学特征。通过使用聚合酶链式反应，我们已经证明人类神经激肽-3受体的mRNA主要在中枢神经系统中表达。
The complete amino acid sequence of the human neurokinin-3 receptor was deduced by DNA sequence analysis of human genomic fragments. Comparison of the predicted primary structure with those for the human neurokinin receptors 1 and 2 shows a highly conserved pattern of seven hydrophobic regions with maximum divergence occuring at the amino- and carboxy-termini. The position of intron-exon junctions are identical to those in other reported neurokinin genes. Using a chimeric genomic-cDNA gene, the human NK-3 receptor was expressed in Xenopus laevis oocytes where it mediates membrane conductance changes in response to its agonist, neurokinin B. More significantly, expression of the gene in mammalian cells resulted in detection of receptor binding as well as neurokinin-stimulated calcium mobilization and arachidonic acid release, all displaying the pharmacological characteristics expected of a neurokinin-3 receptor. By using the polymerase chain reaction we have shown that mRNA for the human neurokinin-3 receptor is expressed predominantly in the central nervous system.