Annexin II expression is regulated during mammalian cell cycle.
Annexin II expression is regulated during mammalian cell cycle.
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DOI：
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发表时间：
1993-12
期刊：
影响因子：
11.2
通讯作者：
Y. Chiang;M. Schneiderman;J. Vishwanatha
中科院分区：
文献类型：
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作者：
Y. Chiang;M. Schneiderman;J. Vishwanatha
膜联蛋白II是癌基因和生长因子相关蛋白酪氨酸激酶的底物。膜联蛋白II的表达升高见于不同类型的癌症，最近的证据表明膜联蛋白II在DNA合成和细胞增殖中的作用。在这份报告中，我们表明，膜联蛋白II的水平受到细胞周期调控。不使用药物，通过有丝分裂细胞选择技术选择中国仓鼠卵巢细胞。随着有丝分裂细胞在细胞周期中的同步进展，我们确定了膜联蛋白II mRNA和蛋白的稳态水平。通过脉冲追踪和核糖核酸酶保护分析测量，膜联蛋白II mRNA的半衰期约为2小时。膜联蛋白II mRNA和蛋白质的稳态水平在有丝分裂细胞中很高。当细胞分裂并进入G1期时，膜联蛋白II mRNA和蛋白质的水平都降低了。膜联蛋白II mRNA和蛋白质的新合成发生在G1早期，当细胞进入S期时，膜联蛋白II mRNA和蛋白质的表达达到最大。膜联蛋白II mRNA和蛋白质的稳态水平的逐渐减少发生细胞进展通过S期。在HeLa细胞中获得了类似的结果。在HeLa细胞中，收集在不同的细胞周期阶段，通过逆流离心淘洗，我们观察到膜联蛋白II在G1-S和S-G2细胞的峰值表达。我们得出结论，从我们的结果，膜联蛋白II的表达在哺乳动物细胞周期的调节。
Annexin II is a substrate for oncogene and growth factor-associated protein-tyrosine kinases. Elevated expression of annexin II is seen in different types of cancers and recent evidence indicates a role for annexin II in DNA synthesis and cell proliferation. In this report we show that the level of annexin II is subject to cell cycle regulation. Chinese hamster ovary cells were selected without the use of drugs, by the mitotic cell selection technique. As the mitotic cells progressed synchronously through the cell cycle, we determined the steady-state levels of annexin II mRNA and protein. The half-life of annexin II mRNA was approximately 2 h as measured by pulse-chase and ribonuclease protection analyses. Steady-state levels of both annexin II mRNA and protein were high in mitotic cells. As the cells divided and entered G1, there was a reduction in the levels of both annexin II mRNA and protein. New synthesis of annexin II mRNA and protein occurred in early G1 and maximal expression of annexin II mRNA and protein occurred as the cells entered S-phase. A gradual reduction in steady-state levels of annexin II mRNA and protein occurred as cells progressed through S-phase. Similar results were obtained in HeLa cells. In HeLa cells, collected at various cell cycle stages by countercurrent centrifugal elutriation, we observed peak expression of annexin II in G1-S and S-G2 cells. We conclude from our results that annexin II expression is regulated during the mammalian cell cycle.