Death Receptor 5-Recruited Raft Components Contributes to the Sensitivity of Jurkat Leukemia Cell Lines to TRAIL-induced Cell Death
Death Receptor 5-Recruited Raft Components Contributes to the Sensitivity of Jurkat Leukemia Cell Lines to TRAIL-induced Cell Death
复制标题
死亡受体 5 招募的筏成分有助于 Jurkat 白血病细胞系对 TRAIL 诱导的细胞死亡的敏感性。
DOI：
10.1002/iub.166
复制
发表时间：
2009-03-01
期刊：
影响因子：
4.6
通讯作者：
Zheng, Dexian
中科院分区：
文献类型：
--
作者：
Min, Yifan;Shi, Juan;Zheng, Dexian
在本研究中，我们证明了TIB 152和TIB 153的Jurkat白血病细胞系对重组可溶性TRAIL细胞毒性具有不同的敏感性。TRAIL受体死亡受体5（DR 5）组成型定位于两种细胞系的筏。FADD、caspase-8和PI 3 K-p85亚基被募集到TIB 152的DR 5脂筏中，但在TIB 153细胞中没有。酸性鞘磷脂酶的表达和酶活性在TIB 152的脂筏中高于TIB 153，该酶抑制鞘磷脂产生神经酰胺并在脂筏组装中起重要作用。这些数据提供的证据表明，DR 5招募筏组件有助于不同的敏感性Jurkat白血病细胞系的TRAIL诱导的细胞死亡，并可能为耐TRAIL治疗的癌细胞的更好的治疗策略的发展带来一些光。(C)2009年IUBMB
In the present study we demonstrated Jurkat leukemia cell lines of TIB152 and TIB153 with different sensitivities to recombinant soluble TRAIL cytotoxicity. TRAIL receptor death receptor 5 (DR5) was constitutively localized in the rafts in both cell lines. FADD, caspase-8, and PI3K-p85 subunit were recruited into DR5 lipid rafts of TIB152 but not in TIB153 cells. The expression and enzyme activity of acid sphingomyelinase, which digests sphingomyeline to produce ceramide and plays an essential role in lipid raft assembling, were higher in the rafts of TIB152 than in TIB153. These data provide evidences that DR5-recruited raft components contribute to the different sensitivity of Jurkat leukemia cell lines to TRAIL-induced cell death and may throw some light on the development of better therapeutic strategies for the cancer cells resistant to TRAIL treatment. (C) 2009 IUBMB