MEASUREMENT OF THE MUTATION-RATES OF ANIMAL VIRUSES - INFLUENZA-A VIRUS AND POLIOVIRUS TYPE-1
MEASUREMENT OF THE MUTATION-RATES OF ANIMAL VIRUSES - INFLUENZA-A VIRUS AND POLIOVIRUS TYPE-1
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DOI：
10.1128/jvi.59.2.377-383.1986
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发表时间：
1986-08-01
影响因子：
5.4
通讯作者：
PALESE, P
中科院分区：
文献类型：
--
作者：
PARVIN, JD;MOSCONA, A;PALESE, P
流行病学和遗传学证据表明，甲型流感病毒在人类体内的进化速度比其他病毒更快。虽然病毒的高突变率经常被认为是广泛变异的原因，但还没有在体内直接测量这一参数。本研究用直接测序法检测了甲型流感病毒非结构基因(NS)和1型脊髓灰质炎病毒VP1基因在组织培养中的突变率。在100多个病毒克隆中对每个基因进行了重复测序，这些克隆是在一个斑块世代中从单个病毒粒子下来的。共测定了108个流感病毒NS基因的序列，在分析的91,708个核苷酸中，观察到7个点的变化。共测定了105个脊髓灰质炎病毒VP1基因的序列，在分析的95,688个核苷酸中，没有观察到突变。然后我们计算了1.5倍的突变率。10-5和不到2.1倍。对于流感病毒和脊髓灰质炎病毒，每个感染周期每个核苷酸分别有10-6个突变。我们认为，甲型流感病毒较高的突变率可能促进了该病毒在自然界中的快速进化。
Epidemiologic and genetic evidence suggests that influenza A viruses evolve more rapidly than other viruses in humans. Although the high mutation rate of the virus is often cited as the cause of the extensive variation, direct measurement of this parameter has not been obtained in vivo. In this study, the rate of mutation in tissue culture for the nonstructural (NS) gene of influenza A virus and for the VP1 gene in poliovirus type 1 was assayed by direct sequence analysis. Each gene was repeatedly sequenced in over 100 viral clones which were descended from a single virion in one plaque generation. A total of 108 NS genes of influenza virus were sequenced, and in the 91,708 nucleotides analyzed, seven point changes were observed. A total of 105 VP1 genes of poliovirus were sequenced, and in the 95,688 nucleotides analyzed, no mutations were observed. We then calculated mutation rates of 1.5 .times. 10-5 and less than 2.1 .times. 10-6 mutations per nucleotide per infectious cycle for influenza virus and poliovirus, respectively. We suggest that the higher mutation rate of influenza A virus may promote the rapid evolution of this virus in nature.