Induction and suppression of RNA silencing by an animal virus
Induction and suppression of RNA silencing by an animal virus
复制标题
DOI：
10.1126/science.1070948
复制
发表时间：
2002-05-17
期刊：
影响因子：
56.9
通讯作者：
Ding, SW
中科院分区：
文献类型：
--
作者：
Li, HW;Li, WX;Ding, SW
RNA沉默是一种序列特异性的RNA降解机制，在植物和动物中起作用。在这里，我们表明，鸡舍病毒（FHV）是一个启动子和果蝇宿主细胞中的RNA沉默的目标，FHV感染需要抑制RNA沉默的FHV编码的蛋白质，B2。这些发现确立了RNA沉默作为动物细胞中的适应性抗病毒防御。B2还抑制转基因植物中的RNA沉默，为植物和动物王国中保守的RNA沉默途径提供了证据。
RNA silencing is a sequence-specific RNA degradation mechanism that is operational in plants and animals. Here, we show that flock house virus (FHV) is both an initiator and a target of RNA silencing in Drosophila host cells and that FHV infection requires suppression of RNA silencing by an FHV-encoded protein, B2. These findings establish RNA silencing as an adaptive antiviral defense in animal cells. B2 also inhibits RNA silencing in transgenic plants, providing evidence for a conserved RNA silencing pathway in the plant and animal kingdoms.