Quantitative expression profile of androgen-regulated genes in prostate cancer cells and identification of prostate-specific genes
Quantitative expression profile of androgen-regulated genes in prostate cancer cells and identification of prostate-specific genes
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DOI：
10.1002/ijc.1196
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发表时间：
2001-05-01
影响因子：
6.4
通讯作者：
Srivastava, S
中科院分区：
文献类型：
--
作者：
Xu, LL;Su, YP;Srivastava, S
通过基因表达系列分析（SAGE）对前列腺癌细胞系LNCaP中雄激素调节基因（ARG）的定量表达谱进行了评估。总共83，489个SAGE标签代表23，448个已知基因或表达序列标签（EST）和1，655个潜在的新序列，已经解开了LNCaP细胞的转录组。前列腺癌研究中最常用的细胞系，对照和R1881处理的LNCaP细胞之间的转录物的比较揭示了响应于雄激素的136个基因的诱导和215个基因的抑制（p < 0.05），引人注目的是，高比例（90%）。在我们的研究中发现的ARGs以前没有被描述为ARGs。一些前列腺特异性转录因子在这里确定的ARG，分类的基础上的ARG的生化功能显示，在我们的实验系统中确定的ARG的绝大多数似乎参与调节转录，剪接，核糖体生物合成，有丝分裂，生物能量学和氧化还原过程。雄激素信号转导的新方面之一包括雄激素对DNA修复/重组过程中相关基因的调节。通过将我们的LNCaP-C和LNCaP-T SAGE文库与NCBI-SAGE网站上可获得的SAGE标签文库进行比较，我们已经鉴定了>200种潜在的前列腺特异性/丰富的转录物。新的前列腺特异性基因和ARG的发现为确定这些基因在前列腺细胞生长、分化和肿瘤发生中的作用提供了独特的机会。(C)2001年威利-利斯。Inc.
Quantitative expression profile of androgen-regulated genes (ARGs) was evaluated in the hormone-responsive prostate cancer cell line LNCaP by serial analysis of gene expression (SAGE), A total of 83,489 SAGE tags representing 23,448 known genes or expressed sequence tags (ESTs) and 1,655 potentially novel sequences have unraveled the transcriptome of LNCaP cells. the most common cell line used in prostate cancer research, Comparison of transcripts between control and R1881-treated LNCaP cells revealed the induction of 136 genes and repression of 215 genes in response to androgen (p < 0.05), Strikingly, a high fraction (90%.) of ARGs identified in our study has not been described as ARGs previously. A number of prostate-specific transcription factors were among the ARGs identified here, Classification of the ARGs on the basis of biochemical functions revealed that a great majority of ARGs identified in our experimental system appear to be involved in regulation of transcription, splicing, ribosomal biogenesis, mitogenesis, bioenergetics and redox processes. One of the novel aspects of androgen signaling included androgen regulation of genes involved in DNA repair/recombination process. By comparing our LNCaP-C and LNCaP-T SAGE libraries with SAGE tag libraries available at the NCBI-SAGE website, we have identified >200 potential prostate specific/abundant transcripts. The discovery of new prostate-specific genes and ARGs provides a unique opportunity to determine the role of these genes in prostate cell growth, differentiation and tumorigenesis. (C) 2001 Wiley-Liss. Inc.