A tissue-engineered conduit for peripheral nerve repair
A tissue-engineered conduit for peripheral nerve repair
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DOI：
10.1001/archotol.124.10.1081
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发表时间：
1998-10-01
影响因子：
--
通讯作者：
Cheney, M
中科院分区：
文献类型：
--
作者：
Hadlock, T;Elisseeff, J;Cheney, M
背景：使用自体移植材料进行周围神经修复有几个缺点，包括供体部位发病率、功能恢复不足和再生异常。最近，周围神经研究的重点是合成神经引导导管的产生，该导管可能克服这些现象以改善再生。在我们的实验室中，我们利用合成聚合物独特的化学和物理特性，结合施万细胞的生物学特性，创建了一种用于修复多分支周围神经（例如面神经）的优质假体。目标：创建一种近似颞外面神经束状结构的聚合物面神经模拟物，将一群施万细胞引入模拟物中，并将假体植入动物模型中以评估再生情况。结果：聚左旋乳酸管（分子量，100000）或聚乳酸-乙醇酸共聚物是使用浸塑技术形成的。它们被创建为包含 1、2、4 或 5 个亚腔，或“束状类似物”。雪旺细胞群被分离出来，在培养物中扩增，并铺在这些聚合物薄膜上，它们在聚合物表面上表现出良好的粘附性。通过几种构造证明了再生。结论：创建了具有多束周围神经宏观结构的管状神经引导导管。证明了驻留雪旺细胞在聚-L-乳酸和聚乳酸-乙醇酸表面上的建立，并显示了通过导管进行体内再生的可行性。据推测，这些组织工程装置由广泛使用的生物相容性、可生物降解的聚合物材料和粘附的雪旺细胞组成，将有助于促进更强大和更精确定向的周围神经再生。
Background: Peripheral nerve repair using autograft material has several shortcomings, including donor site morbidity, inadequate return of function, and aberrant regeneration. Recently, peripheral nerve research has focused on the generation of synthetic nerve guidance conduits that might overcome these phenomena to improve regeneration. In our laboratory, we use the unique chemical and physical properties of synthetic polymers in conjunction with the biological properties of Schwann cells to create a superior prosthesis for the repair of multiply branched peripheral nerves, such as the facial nerve.Objectives: To create a polymeric facial nerve analog approximating the fascicular architecture of the extratemporal facial nerve, to introduce a population of Schwann cells into the analog, and to implant the prosthesis into an animal model for assessment of regeneration.Results: Tubes of poly-L-lactic acid (molecular weight, 100000) or polylactic-co-glycolic acid copolymer were formed using a dip-molding technique. They were created containing 1, 2, 4, or 5 sublumina, or "fascicular analogs." Populations of Schwann cells were isolated, expanded in culture, and plated onto these polymer films, where they demonstrated excellent adherence to the polymer surfaces. Regeneration was demonstrated through several constructs.Conclusions: A tubular nerve guidance conduit possessing the macroarchitecture of a polyfascicular peripheral nerve was created. The establishment of resident Schwann cells onto poly-L-lactic acid and polylactic-co-glycolic acid surfaces was demonstrated, and the feasibility of in vivo regeneration through the conduit was shown. It is hypothesized that these tissue-engineered devices, composed of widely used biocompatible, biodegradable polymer materials and adherent Schwann cells, will be useful in promoting both more robust and more precisely directed peripheral nerve regeneration.