COMPREHENSIVE CHEMICAL MODIFICATION INTERFERENCE AND NUCLEOTIDE SUBSTITUTION ANALYSIS OF AN RNA PSEUDOKNOT INHIBITOR TO HIV-1 REVERSE-TRANSCRIPTASE
COMPREHENSIVE CHEMICAL MODIFICATION INTERFERENCE AND NUCLEOTIDE SUBSTITUTION ANALYSIS OF AN RNA PSEUDOKNOT INHIBITOR TO HIV-1 REVERSE-TRANSCRIPTASE
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DOI：
10.1006/jmbi.1994.0122
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发表时间：
1995-03-17
影响因子：
5.6
通讯作者：
TUERK, C
中科院分区：
文献类型：
--
作者：
GREEN, L;WAUGH, S;TUERK, C
我们以前使用体外RNA选择技术来描述一个共识的RNA假结，它结合并抑制HTV-1逆转录酶(HIV-RT)。在这项工作中，我们构建了这个共识假结的变体，以评估单个核苷酸同一性和二级结构对HIV-RT亲和力的贡献。我们还使用了配体RNA的化学修饰来证实假结的预测结构，发现哪些可修饰基团在与HIV-RT结合时受到化学攻击，以及哪些修饰干扰了与HIV-RT的结合。提出了一种新的干扰研究，包括从混合试剂寡核苷酸合成产生的池中选择配体，以便快速确定此类RNA配体中2‘-OCH3基团对通常的2’-OH基团的允许取代。
We had previously used in vitro RNA selection techniques to describe a consensus RNA pseudoknot that binds and inhibits HTV-1 reverse transcriptase (HIV-RT). In this work we constructed variants of this consensus pseudoknot in order to evaluate the contributions of individual nucleotide identities and secondary structure to affinity for HIV-RT. We have also used chemical modification of Ligand RNAs to corroborate the predicted structure of the pseudoknot, to discover which modifiable groups are protected from chemical attack when bound to HIV-RT, and to find which modifications interfere with binding to HIV-RT. A novel interference study is presented which involves selection of ligands from a pool created by mixed reagent oligonucleotide synthesis in order to rapidly determine allowed substitutions of 2'-OCH3 groups for the usual 2'-OH group in such RNA ligands.