Protein oxidation by the cytochrome P450 mixed-function oxidation system.
Protein oxidation by the cytochrome P450 mixed-function oxidation system.
复制标题
细胞色素 P450 混合功能氧化系统对蛋白质进行氧化。
DOI：
10.1016/j.bbrc.2005.07.203
复制
发表时间：
2005
影响因子：
3.1
通讯作者：
Berlett,BarbaraS
中科院分区：
文献类型：
--
作者：
Stadtman,EarlR;Arai,Hirofumi;Berlett,BarbaraS
这篇小综述总结了各种混合功能氧化 (MFO) 系统对蛋白质和低密度脂蛋白 (LDL) 氧化的研究结果。 O2/FeCl3/H2O2/抗坏血酸 MFO 系统对 LDL 的氧化取决于所有四种组分，并且与磷酸盐缓冲液相比，在生理性碳酸氢盐/CO2 缓冲液系统存在下进行反应时氧化程度要大得多。然而，该系统中的 FeCl3 可以被血红素或含血红素的蛋白质、血红蛋白或细胞色素 c 取代。与磷酸盐缓冲液相比，碳酸氢盐/CO2 缓冲液中 O2/细胞色素 P450 细胞色素 c 还原酶/NADPH/FeCl3MFO 系统对 LDL 的氧化仅略高 (25%)，但取决于除 FeCl3 之外的所有成分。 FeCl3 的省略导致活性损失 60%。这些结果表明，过一碳酸氢盐和/或碳酸氢根离子和/或CO2的自由基衍生物可能有助于这些MFO系统对LDL的氧化。
This mini-review summarizes results of studies on the oxidation of proteins and low-density lipoprotein (LDL) by various mixed-function oxidation (MFO) systems. Oxidation of LDL by the O2/FeCl3/H2O2/ascorbate MFO system is dependent on all four components and is much greater when reactions are carried out in the presence of a physiological bicarbonate/CO2buffer system as compared to phosphate buffer. However, FeCl3in this system could be replaced by hemin or the heme-containing protein, hemoglobin, or cytochrome c. Oxidation of LDL by the O2/cytochrome P450 cytochrome c reductase/NADPH/FeCl3MFO system is only slightly higher (25%) in the bicarbonate/CO2buffer as compared to phosphate buffer, but is dependent on all components except FeCl3. Omission of FeCl3led to a 60% loss of activity. These results suggest that peroxymonobicarbonate and/or free radical derivatives of bicarbonate ion and/or CO2might contribute to LDL oxidation by these MFO systems.