PDMS-glass hybrid microreactor array with embedded temperature control device. Application to cell-free protein synthesis
PDMS-glass hybrid microreactor array with embedded temperature control device. Application to cell-free protein synthesis
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DOI：
10.1039/b205010b
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发表时间：
2002-01-01
期刊：
影响因子：
6.1
通讯作者：
Fujii, T
中科院分区：
文献类型：
--
作者：
Yamamoto, T;Nojima, T;Fujii, T
开发了一种微反应器阵列，使高通量无细胞蛋白质合成成为可能。微反应器阵列由温控芯片和反应室芯片组成。温控芯片是一种玻璃制造的芯片，其上的温度控制设备、加热器和温度传感器都是用ITO(氧化铟锡)电阻材料制造的。反应室芯片由聚二甲基硅氧烷(PDMS)微模成型而成，设计成具有一系列反应室和用于液体导入的流动通道。通过将反应室芯片放置在温度控制芯片上来组装微反应器阵列。由于反应室的热质量较小，导致加热的热时间常数较短，为170ms，冷却的热时间常数为3 S。通过无细胞蛋白质合成实验对微反应器阵列的性能进行了检验。通过检测产物的荧光发射，证实利用大肠杆菌提取液成功合成了绿色荧光蛋白(GFP)和蓝色荧光蛋白(BFP)。
A microreactor array was developed which enables high-throughput cell-free protein synthesis. The microreactor array is composed of a temperature control chip and a reaction chamber chip. The temperature control chip is a glass-made chip on which temperature control devices, heaters and temperature sensors, are fabricated with an ITO (indium tin oxide) resistive material. The reaction chamber chip is fabricated by micromolding of PDMS (polydimethylsiloxane), and is designed to have an array of reaction chambers and flow channels for liquid introduction. The microreactor array is assembled by placing the reaction chamber chip on the temperature control chip. The small thermal mass of the reaction chamber resulted in a short thermal time constant of 170 ms for heating and 3 s for cooling. The performance of the microreactor array was examined through the experiments of cell-free protein synthesis. By measuring the fluorescence emission from the products, it was confirmed that GFP (Green Fluorescent Protein) and BFP (Blue Fluorescent Protein) were successfully synthesized using Escherichia coli extract.