Neurochemically distinct classes of myenteric neurons express the μ-opioid receptor in the guinea pig ileum
Neurochemically distinct classes of myenteric neurons express the μ-opioid receptor in the guinea pig ileum
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DOI：
10.1002/cne.10606
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发表时间：
2003-04-14
影响因子：
2.5
通讯作者：
Sternini, C
中科院分区：
文献类型：
--
作者：
Ho, A;Lievore, A;Sternini, C
多阿片受体（mu-opioid receptor， LOR）介导了许多神经系统的阿片效应，它是由肠神经元表达的。本研究的目的是通过双标记和三标记免疫荧光和共聚焦显微镜来确定1)豚鼠回肠不同类型的肌肠神经元是否具有muOR免疫反应性，2)muOR免疫反应性是否定位于内源性阿片脑啡肽免疫反应的肠神经元，3)muOR免疫反应性是否定位于c-kit可视化的Cajal间质细胞。在肌肠丛中，50%的muor免疫反应神经元具有胆碱乙酰转移酶（ChAT）免疫反应性，而约43%的ChAT免疫反应性神经元具有胆碱乙酰转移酶免疫反应性。约46%的muOR肌肠神经元对血管活性肠多肽（VIP）有免疫反应，约31%对一氧化氮合酶（NOS）有免疫反应。约68%的vip神经元和60%的nos神经元具有muOR免疫反应性。三重标记显示约32%的muOR神经元具有VIP和ChAT免疫反应。内源性阿片样脑啡肽（ENK）存在于约30%的muOR神经元中；相反，48%的ENK神经元具有muOR免疫反应性。在含有calbindin的神经元和Cajal间质细胞中均未检测到muOR。muor免疫反应纤维在深肌丛Cajal间质细胞周围形成致密的网络。这项研究表明，muOR是由神经化学上不同类别的肌肠神经元表达的，这些神经元可能具有不同的功能，与内源性阿片样物质ENK共定位，而不是由Cajal间质细胞表达。(C) 2003 Wiley-Liss, Inc。
The mu-opioid receptor ( LOR), which mediates many of the opioid effects in the nervous system, is expressed by enteric neurons. The aims of this study were to determine whether 1) different classes of myenteric neurons in the guinea pig ileum contain muOR immunoreactivity by using double- and triple-labeling immunofluorescence and confocal microscopy, 2) muOR immunoreactivity is localized to enteric neurons immunoreactive for the endogenous opioid enkephalin, and 3) muOR immunoreactivity is localized to interstitial cells of Cajal visualized by c-kit. In the myenteric plexus, 50% of muOR-immunoreactive neurons contained choline acetyltransferase (ChAT) immunoreactivity, whereas about 43% of ChAT-immunoreactive neurons were LOR immunoreactive. Approximately 46% of muOR myenteric neurons were immunoreactive for vasoactive intestinal polypeptide (VIP), and about 31% were immunoreactive for nitric oxide synthase (NOS). muOR immunoreactivity was found in about 68% of VIP-containing neurons and 60% of NOS-immunoreactive neurons. Triple labeling showed that about 32% of muOR neurons contained VIP and ChAT immunoreactivities. The endogenous opioid enkephalin (ENK) was observed in about 30% of muOR neurons; conversely, 48% of ENK neurons contained muOR immunoreactivity. muOR was not detected in neurons containing calbindin, nor in interstitial cells of Cajal. muOR-immunoreactive fibers formed a dense network around interstitial cells of Cajal in the deep muscular plexus. This study demonstrates that muOR is expressed by neurochemically distinct classes of myenteric neurons that are likely to differ functionally, is colocalized with the endogenous opioid ENK, and is not expressed by interstitial cells of Cajal. (C) 2003 Wiley-Liss, Inc.