Concerted control of the LaRAV1-LaCDKB1;3 module by temperature during dormancy release and reactivation of larch.
Concerted control of the LaRAV1-LaCDKB1;3 module by temperature during dormancy release and reactivation of larch.
复制标题
落叶松休眠释放和重新激活期间温度对 LaRAV1-LaCDKB1;3 模块的协同控制
DOI：
10.1093/treephys/tpab052
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发表时间：
2021-10-04
期刊：
影响因子：
4
通讯作者：
Qi LW
中科院分区：
文献类型：
--
作者：
Li WF;Kang Y;Zhang Y;Zang QL;Qi LW
温带树木休眠的解除和再激活涉及细胞周期基因的温度调控表达。然而，关于详细的监管机制的信息有限。在这里，我们比较了活跃和休眠落叶松茎的转录组，强调了细胞周期基因和转录因子的表达模式，并评估了它们的关系和对温度的反应。12个细胞周期基因和31个转录因子在活跃期表达较强。启动子分析表明，这12个基因可能受到来自10个家族的转录因子的调控。共预测了16个转录因子和12个细胞周期基因之间的73个调控案例，而LaMYB 20和LaCYCB 1;1，LaRAV 1和LaCDKB 1;3之间的调控相互作用通过酵母单杂交和双荧光素酶测定得到证实。最后，我们发现LaRAV 1和LaCDKB 1;3在自然和人工温度诱导的落叶松休眠解除和再激活过程中具有几乎相同的表达模式，表明LaRAV 1-LaCDKB 1;3模块在落叶松休眠解除和再激活的温度调控中发挥作用。这些结果为温度和细胞周期基因表达之间的联系提供了新的见解，有助于了解气候变化背景下树木生长和发育的温度控制。
Abstract Dormancy release and reactivation of temperate-zone trees involve the temperature-modulated expression of cell-cycle genes. However, information on the detailed regulatory mechanism is limited. Here, we compared the transcriptomes of the stems of active and dormant larch trees, emphasizing the expression patterns of cell-cycle genes and transcription factors and assessed their relationships and responses to temperatures. Twelve cell-cycle genes and 31 transcription factors were strongly expressed in the active stage. Promoter analysis suggested that these 12 genes might be regulated by transcription factors from 10 families. Altogether, 73 cases of regulation between 16 transcription factors and 12 cell-cycle genes were predicted, while the regulatory interactions between LaMYB20 and LaCYCB1;1, and LaRAV1 and LaCDKB1;3 were confirmed by yeast one-hybrid and dual-luciferase assays. Last, we found that LaRAV1 and LaCDKB1;3 had almost the same expression patterns during dormancy release and reactivation induced naturally or artificially by temperature, indicating that the LaRAV1-LaCDKB1;3 module functions in the temperature-modulated dormancy release and reactivation of larch trees. These results provide new insights into the link between temperature and cell-cycle gene expression, helping to understand the temperature control of tree growth and development in the context of climate change.