Nerve growth factor loaded heparin/chitosan scaffolds for accelerating peripheral nerve regeneration
Nerve growth factor loaded heparin/chitosan scaffolds for accelerating peripheral nerve regeneration
复制标题
负载神经生长因子的肝素/壳聚糖支架加速周围神经再生
DOI：
10.1016/j.carbpol.2017.05.006
复制
发表时间：
2017-09-01
影响因子：
11.2
通讯作者：
Yang, Yumin
中科院分区：
文献类型：
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作者：
Li, Guicai;Xiao, Qinzhi;Yang, Yumin
人工壳聚糖支架在周围神经再生方面已得到广泛研究。但其效果不如自体移植，不能满足临床需要。为了进一步促进神经再生，本研究利用静电相互作用制备了神经生长因子（NGF）负载肝素/壳聚糖支架。测试了其形貌、润湿性和组成等物理化学性能。分别对肝素固定、NGF负载和释放进行了定量和定性表征。采用雪旺细胞培养法观察不同时间的肝素/壳聚糖复合支架对神经再生的影响。结果表明，肝素固定和NGF负载并没有引起壳聚糖支架的整体性质的变化，除了形态和润湿性的变化。肝素在壳聚糖支架中的预固定可以提高后续负载NGF的稳定性。肝素/壳聚糖复合支架能明显促进雪旺细胞的贴壁和增殖。更重要的是，载NGF的肝素/壳聚糖支架能有效促进雪旺细胞的形态发育。本研究为周围神经再生及其他组织再生人工植入物的设计和开发提供了实验依据。(C)2017爱思唯尔有限公司版权所有
Artificial chitosan scaffolds have been widely investigated for peripheral nerve regeneration. However, the effect was not as good as that of autologous grafts and therefore could not meet the clinical requirement. In the present study, the nerve growth factor (NGF) loaded heparin/chitosan scaffolds were fabricated via electrostatic interaction for further improving nerve regeneration. The physicochemical properties including morphology, wettability and composition were measured. The heparin immobilization, NGF loading and release were quantitatively and qualitatively characterized, respectively. The effect of NGF loaded heparin/chitosan scaffolds on nerve regeneration was evaluated by Schwann cells culture for different periods. The results showed that the heparin immobilization and NGF loading did not cause the change of bulk properties of chitosan scaffolds except for morphology and wettability. The pre-immobilization of heparin in chitosan scaffolds could enhance the stability of subsequently loaded NGF. The NGF loaded heparin/chitosan scaffolds could obviously improve the attachment and proliferation of Schwann cells in vitro. More importantly, the NGF loaded heparin/chitosan scaffolds could effectively promote the morphology development of Schwann cells. The study may provide a useful experimental basis to design and develop artificial implants for peripheral nerve regeneration and other tissue regeneration. (C) 2017 Elsevier Ltd. All rights reserved.