The effects of processing variables on electrospun poly(ethylene glycol) fibrous hydrogels formed from the thiol‐norbornene click reaction
The effects of processing variables on electrospun poly(ethylene glycol) fibrous hydrogels formed from the thiol‐norbornene click reaction
复制标题
工艺变量对硫醇-降冰片烯点击反应形成的电纺聚乙二醇纤维水凝胶的影响
DOI：
10.1002/app.50786
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发表时间：
2021
影响因子：
3
通讯作者：
Bryant, Stephanie J.
中科院分区：
文献类型：
--
作者：
Sharma, Sadhana;Monteleone, Nicholas;Kopyeva, Irina;Bryant, Stephanie J.
静电纺丝已被用于创建具有可调微/纳米结构、刚度和孔隙率的支架，以模拟天然细胞外基质。本研究调查了静电纺丝参数和水凝胶配方（溶剂和交联剂类型）对由光点击巯基-异戊二烯反应形成的纤维状聚（乙二醇）（PEG）水凝胶的结构和性能的影响。使用水凝胶前体（四臂PEG双烯和多硫醇交联剂）、牺牲性聚（环氧乙烷）（PEO，400 kDa）和光引发剂（I2959）在2，2-三氟乙醇（TFE）或水中制备纤维水凝胶。研究了三种硫醇交联剂2，2 ′-（亚乙基二氧基）二乙撑二醇（EDT）、季戊四醇四（3-巯基丙酸酯）（PTMP）和PEG二硫醇（PEGDT）。具有均匀纤维的纤维状PEG网络在对于TFE为10或12 kV和对于水为16 kV的施加电压下产生。当与PEO浓度结合时，静电纺丝水凝胶的纤维直径受溶剂的影响很大，并且在干燥状态下在0.5至3.5 mm之间。虽然交联剂类型对纤维水凝胶的纤维直径、形态和孔隙率的影响是最小的，但它确实调节其剪切模量。为此，本研究为选择工艺参数提供了基础，以实现纤维PEG硫醇-藜芦烯水凝胶的预期性能，用于从神经，心血管到肌肉骨骼的组织工程应用。
Electrospinning has been used to create scaffolds with tunable micro/nano architecture, stiffness, and porosity to mimic native extracellular matrix. This study investigated the effects of electrospinning parameters and hydrogel formulation (solvent and crosslinker type) on the architecture and properties of fibrous poly(ethylene glycol) (PEG) hydrogels formed from a photoclick thiol‐norbornene reaction. Fibrous hydrogels were prepared using hydrogel precursors (four‐arm PEG norbornene and multi‐thiol crosslinker), sacrificial poly(ethylene oxide) (PEO, 400 kDa), and photoinitiator (I2959) in either 2,2‐triflouroethanol (TFE) or water. Three thiol crosslinkers‐ 2,2′‐(ethylenedioxy)diethanethiol (EDT), pentaerythritol tetrakis(3mercaptopropionate) (PTMP), and PEG dithiol (PEGDT)‐ were investigated. Fibrous PEG networks with uniform fibers were produced at applied voltages of 10 or 12 kV for TFE and 16 kV for water. Fiber diameters of electrospun hydrogels were largely affected by the solvent when combined with PEO concentration and ranged from 0.5 to 3.5 mm in dry state. While the effect of crosslinker type on fiber diameter, morphology, and porosity of the fibrous hydrogel was minimal, it did modulate its shear modulus. To this end, this study provides the groundwork for selecting processing parameters to achieve desired properties of fibrous PEG thiol‐norbornene hydrogels for intended tissue engineering applications ranging from neural, cardiovascular to musculoskeletal.