Changes in the pattern of adherens junction-associated beta-catenin accompany morphogenesis in the sea urchin embryo.
Changes in the pattern of adherens junction-associated beta-catenin accompany morphogenesis in the sea urchin embryo.
复制标题
海胆胚胎中粘附连接相关的β-连环蛋白模式的变化伴随着形态发生。
DOI：
10.1006/dbio.1997.8739
复制
发表时间：
1997
影响因子：
2.7
通讯作者：
McClay,DR
中科院分区：
文献类型：
--
作者：
Miller,JR;McClay,DR
β-连环蛋白最初在生物化学上被鉴定为在培养细胞中结合E-钙粘蛋白的蛋白质，并且这种相互作用后来被证明是钙粘蛋白功能所必需的。另外，果蝇β-catenin的同源基因armadillo被鉴定为一个在胚胎和幼虫发育过程中传递无翅信号（Wnt）所必需的体节极性基因。最近，一些研究也表明β-catenin在早期非洲爪蟾、斑马鱼和小鼠胚胎的轴向模式中起着关键作用。在这些系统中，β-连环蛋白定位于质膜、胞质溶胶和细胞核可预测其在细胞粘附和信号传导中的作用。为了研究β-catenin在胚胎发生过程中调节细胞粘附的潜在作用，我们克隆了海胆Lytechinus variegatus中的β-catenin，并表征了其在经历形态发生运动的细胞中的亚细胞分布。表明在细胞粘附的建立和维持中的作用，β-连环蛋白与侧向细胞-细胞接触相关，并从卵裂阶段开始在粘附连接处积累。在原肠胚形成时，连接β-连环蛋白定位的变化伴随着几个形态发生事件。上皮-间充质转化是原发性和继发性间充质细胞侵入的特征，与粘附连接相关的β-连环蛋白的快速和急剧丧失相一致。此外，当原肠上皮细胞进行会聚-伸展运动时，其粘附连接相关β-连环蛋白水平显著降低。这些数据与β-连环蛋白在海胆胚胎原肠胚形成过程中调节细胞粘附和粘附连接功能的作用一致。
β-Catenin was originally identified biochemically as a protein that binds E-cadherin in cultured cells and that interaction was later shown to be essential for cadherin function. Independently,armadillo,the β-catenin homolog inDrosophila melanogaster,was identified as a segment polarity gene necessary for the transduction ofwingless (Wnt)signals during embryonic and larval development. Recently, several investigations have also shown that β-catenin plays a critical role in axial patterning of earlyXenopus,zebrafish, and mouse embryos. In these systems, the localization of β-catenin to the plasma membrane, cytosol, and nucleus is predictive of its role in cell adhesion and signaling. In order to examine the potential role of β-catenin in regulating cell adhesion during embryogenesis, we cloned β-catenin in the sea urchinLytechinus variegatusand characterized its subcellular distribution in cells undergoing morphogenetic movements. Indicative of a role in the establishment and maintenance of cell adhesion, β-catenin is associated with lateral cell–cell contacts and accumulates at adherens junctions from cleavage stages onward. At gastrulation, changes in junctional β-catenin localization accompany several morphogenetic events. The epithelial–mesenchymal conversion that characterizes the ingression of both primary and secondary mesenchyme cells coincides with a rapid and dramatic loss of adherens junction-associated β-catenin. In addition, epithelial cells in the archenteron display a significant decrease in adherens junction-associated β-catenin levels as they undergo convergent–extension movements. These data are consistent with a role for β-catenin in regulating cell adhesion and adherens junction function during gastrulation in the sea urchin embryo.