Endothelial mitosis during the initial stages of corpus luteum neovascularization in the cycling adult rat.
Endothelial mitosis during the initial stages of corpus luteum neovascularization in the cycling adult rat.
复制标题
循环成年大鼠黄体新生血管初始阶段的内皮有丝分裂。
DOI：
--
复制
发表时间：
1985
影响因子：
--
通讯作者：
S. Quattropani
中科院分区：
文献类型：
--
作者：
S. Gaede;M. Sholley;S. Quattropani
用光镜放射自显影技术研究了成年大鼠周期黄体新生血管形成的早期阶段。这项分析的目的是确定内皮细胞有丝分裂是否是血管生长的一个因素，以及卵巢不同区域的有丝分裂活动量是否存在差异。在排卵后1-2小时和7-8小时两个时间间隔检查卵巢。动物在卵巢灌流固定前20分钟接受了氚胸苷的腹腔注射。放射自显影显示内皮细胞核中的氚胸苷标记被认为是有丝分裂前DNA合成的迹象。在这两个时间间隔内，卵巢中标记的内皮细胞的百分比根据所检查的组织区域和该区域的分化阶段而不同。间质血管的标记较膜血管轻。生长卵泡周围的膜血管比闭锁卵泡周围的膜血管标记更重。最重的标记出现在排卵后7-8小时发育中的黄体。在之前周期中形成的黄体中，最小的标记是明显的。卵巢间皮细胞也存在地区性差异。覆盖在排卵卵泡和发育中的黄体上的间皮细胞所占比例最大。这项研究的主要发现是：在黄体新生血管的初始阶段，内皮细胞有丝分裂增加；内皮细胞标记的增加局限于卵巢的特定区域；靠近发育中的黄体的间皮细胞也显示出DNA合成增加。
The initial stages of neovascularization of the corpus luteum were studied in cycling adult rats using light-microscopic autoradiography. The aim of this analysis was to determine whether endothelial mitosis is a factor in this vascular growth and whether there are differences in the amount of mitotic activity in various regions of the ovary. Ovaries were examined at two time intervals: 1-2 hr and 7-8 hr following ovulation. Animals received an intraperitoneal injection of tritiated-thymidine 20 min prior to perfusion fixation of the ovaries. Autoradiographic demonstration of tritiated-thymidine labeling in endothelial nuclei was considered an indication of DNA synthesis preceding mitosis. The percentage of labeled endothelial cells in the ovaries at both time intervals varied according to the region of tissue examined and the stage of differentiation of that region. Stromal vessels were less heavily labeled than thecal vessels. Thecal vessels surrounding growing follicles were more heavily labeled than those surrounding atretic follicles. The heaviest labeling was seen in the developing corpora lutea 7-8 hr following ovulation. Minimal labeling was evident in the corpora lutea which were formed in previous cycles. A regional difference was also detected in the ovarian mesothelium. The portion of the mesothelium overlying ovulated follicles and developing corpora lutea had the greatest percentage of labeled cells. The major findings of this study were: endothelial mitosis was elevated in the initial stages of luteal neovascularization; the heightened endothelial labeling was confined to specific regions of the ovary; and mesothelium in close proximity to the developing corpora lutea also displayed heightened DNA synthesis.