Cell proliferation and apoptosis in human uterine leiomyomas and myometria
Cell proliferation and apoptosis in human uterine leiomyomas and myometria
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DOI：
10.1007/s00428-001-0568-7
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发表时间：
2002-07-01
期刊：
影响因子：
3.5
通讯作者：
Robboy, SJ
中科院分区：
文献类型：
--
作者：
Dixon, D;Flake, GP;Robboy, SJ
为了确定细胞增殖和凋亡在子宫平滑肌瘤生长中的作用，我们研究了凋亡的两个主要调节蛋白Bcl-2（抗凋亡）和Bax（促凋亡）以及细胞复制的两个内源性标志物增殖细胞核抗原（PCNA）和Ki-67在肿瘤和绝经前妇女子宫肌层中的蛋白表达。常规的有丝分裂指数也进行了测定，所有的增殖数据与肿瘤大小相关。原位末端标记的片段化DNA和常规组织学被用来评估细胞凋亡。我们的研究结果表明，凋亡调节蛋白（Bcl-2和Bax）在整个月经周期的子宫肌瘤和平滑肌细胞的胞浆中表达。Bax的表达与Bcl-2不同，它也被发现在子宫肌层和肿瘤的血管平滑肌细胞的细胞质中。肿瘤和子宫肌层样品均表达26-kDa和21-kDa蛋白，其分别与针对Bcl-2和Bax的抗体反应。细胞凋亡不是子宫肌瘤或子宫肌层的显著特征。PCNA和Ki-67标记及核分裂计数与正常对照组比较差异有显著性（P
To determine the role of cell proliferation and apoptosis in uterine leiomyoma growth, we studied protein expression of two major regulatory proteins of apoptosis - Bcl-2 (anti-apoptotic) and Bax (pro-apoptotic) and two endogenous markers of cell replication - proliferating cell nuclear antigen (PCNA) and Ki-67 - in tumors and matched myometrium from premenopausal women. Conventional mitotic indices also were determined, and all proliferation data were correlated to tumor size. In situ end-labeling of fragmented DNA and routine histology were used to assess apoptosis. Our results showed that the apoptosis-regulating proteins (Bcl-2 and Bax) were expressed in the cytoplasm of the leiomyoma and myometrial smooth muscle cells throughout the menstrual cycle. Bax expression differed from Bcl-2 in that it also was found in the cytoplasm of vascular smooth muscle cells of the myometria and tumors. Both tumors and myometrial samples expressed 26-kDa and 21-kDa proteins that reacted with antibodies directed towards Bcl-2 and Bax, respectively. Apoptosis was not a prominent feature of uterine leiomyomas or myometrium. PCNA- and Ki-67-labeling and mitotic counts were significantly (P