Ecotypes of the model legume Lotus japonicus vary in their interaction phenotypes with the root-knot nematode Meloidogyne incognita.
Ecotypes of the model legume Lotus japonicus vary in their interaction phenotypes with the root-knot nematode Meloidogyne incognita.
复制标题
模式豆科植物百脉根的生态型在与根结线虫根结线虫的相互作用表型方面有所不同。
DOI：
10.1093/aob/mcm058
复制
发表时间：
2007
期刊：
影响因子：
4.2
通讯作者：
S. Clark
中科院分区：
文献类型：
--
作者：
H. L. C. Poch;R. M. López;S. Clark
背景和目标
关于影响植物-线虫相互作用的寄主因素的知识很少。在这里，通过遗传筛选评估了结瘤模式寄主莲花和寄生根结线虫南方根结线虫之间的相关相互作用表型。
方法
在阿尔法实验设计内，从60个日本血吸虫生态型的4周龄复制植株上接种来自单个卵块种群的1000条线虫，并在接种后6周评估粘虫和线虫卵块的情况。
关键结果
对57个生态型数据的统计分析表明，生态型敏感性为3.50~406个/根，与线虫繁殖量(0.6~34.5个/根)密切相关(r=0.80，P<；0.001，对数标度)。然而，一些生态型在疾病严重程度和线虫繁殖之间显示出显著的差异。在其他感染的生态型中也观察到了坏死和发育畸形。
结论
首次提供了日本根结线虫与根结线虫之间相互作用的显著变异性的证据，这可能对线虫寄生以及可能的微生物共生所涉及的寄主途径的遗传解剖和表征具有进一步的意义。
BACKGROUND AND AIMS
Knowledge of host factors affecting plant-nematode interactions is scarce. Here, relevant interaction phenotypes between a nodulating model host, Lotus japonicus, and the endoparasitic root-knot nematode Meloidogyne incognita are assessed via a genetic screen.
METHODS
Within an alpha experimental design, 4-week-old replicate plants from 60 L. japonicus ecotypes were inoculated with 1000 nematodes from a single egg mass population, and evaluated for galling and nematode egg masses 6 weeks after inoculation.
KEY RESULTS
Statistical analysis of data for 57 ecotypes showed that ecotype susceptibilities ranged from 3.5 to 406 galls per root, and correlated strongly (r = 0.8, P < 0.001, log scale) with nematode reproduction (ranging from 0.6 to 34.5 egg masses per root). Some ecotypes, however, showed a significant discrepancy between disease severity and nematode reproduction. Necrosis and developmental malformations were observed in other infected ecotypes.
CONCLUSIONS
The first evidence is provided of significant variability in the interactions between L. japonicus and root-knot nematodes that may have further implications for the genetic dissection and characterization of host pathways involved in nematode parasitism and, possibly, in microbial symbiosis.