Improvement of the tetramethyl benzidine reaction with ammonium molybdate as a stabilizer for light and electron microscopic ligand-HRP neurohistochemistry, immunocytochemistry and double-labelling
Improvement of the tetramethyl benzidine reaction with ammonium molybdate as a stabilizer for light and electron microscopic ligand-HRP neurohistochemistry, immunocytochemistry and double-labelling
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钼酸铵作为光镜配体-HRP 神经组织化学、免疫细胞化学和双标记稳定剂的四甲基联苯胺反应的改进
DOI：
10.1016/0165-0270(89)90031-9
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发表时间：
1989
影响因子：
3
通讯作者：
X. Wan
中科院分区：
文献类型：
--
作者：
Fengyi Liang;X. Wan
以七钼酸铵（AHM）为稳定剂，采用四甲基联苯胺（TMB）标记辣根过氧化物酶（CB-HRP）神经组织化学方法（TMB-AHM法），对霍乱原B亚单位进行标记。与采用硝普钠作为稳定剂的Mesulam的TMB方法（TMB-SNP方法）相比，TMB-AHM程序在CB-HRP标记的神经元及其单核的Golgi-phobic树突的可视化方面提供相似的灵敏度。然而，它是不太敏感的示范顺行运输的CB-HRP轴突终末。在反应介质的接近生理pH值（pH 6-8）下，其证明了反应切片中组织和细胞结构的更好保存。电镜下可清楚地观察到特异性反应产物，细胞及亚细胞结构损伤小。本文还对TMB-AHM法进行了初步应用，并将其应用于霍乱原B亚单位（CB）免疫细胞化学和双标记技术，使HRP神经组织化学和CB免疫细胞化学的神经元示踪方法配对。
Ammonium heptamolybdate (AHM) was used as a stabilizing agent in the tetramethyl benzidine (TMB) reaction of choleragen subunit B conjugated horseradish peroxidase (CB-HRP) neurohistochemistry (TMB-AHM method). In comparison with Mesulam's TMB method employing sodium nitroprusside as a stabilizing agent (TMB-SNP method), the TMB-AHM procedure offfers a similar sensitivity with regard to the visualization of CB-HRP labelled neurons and their extranuclear Golgi-phobic dendrites. However, it is less sensitive for the demonstration of anterogradely transported CB-HRP in axon terminals. At the nearly physiological pH value of the reaction medium (pH 6–8), it demonstrates better preservation of tissue and cell structures in the reacted sections. Under the electron microscope, the specific reaction product can be clearly distinguished and little damage of cellular and subcellular structures occurred. Preliminary application of TMB-AHM method to choleragen subunit B (CB) immunocytochemistry and double labelling technique which paired the neuronal tracing methods of HRP neurohistochemistry and CB immunocytochemistry, was also carried out with small modification.
使用过氧化物酶-抗过氧化物酶方法和单克隆抗体对八种不同的显色剂方案进行比较，以演示大鼠小脑中的免疫反应性神经丝或神经胶质丝。
DOI：
10.1177/31.10.6350434
发表时间：
1983
期刊：
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
影响因子：
--
作者：
Trojanowski,JQ;Obrocka,MA;Lee,VM
通讯作者：
Lee,VM
影响因子：
3.2
作者：
RYE, DB;SAPER, CB;WAINER, BH
通讯作者：
WAINER, BH
联苯胺二盐酸盐作为单标记和双标记光和电子显微镜免疫细胞化学研究的色原。
DOI：
10.1177/34.8.2426333
发表时间：
1986
期刊：
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
影响因子：
--
作者：
Lakos,S;Basbaum,AI
通讯作者：
Basbaum,AI