A comprehensive expression analysis of the starch synthase gene family in rice (Oryza sativa L.)
A comprehensive expression analysis of the starch synthase gene family in rice (Oryza sativa L.)
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DOI：
10.1007/s00425-004-1314-6
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发表时间：
2004-11-01
期刊：
影响因子：
4.3
通讯作者：
Terao, T
中科院分区：
文献类型：
--
作者：
Hirose, T;Terao, T
为阐明编码淀粉合成酶（EC 2.4.1.21）的等基因在水稻中的作用，对该基因家族进行了全面的表达分析。在水稻全基因组和全长cdna数据库中对淀粉合酶基因进行了广泛的检索，发现10个基因属于淀粉合酶基因家族。对水稻和其他植物淀粉合成酶蛋白的多序列比对表明，它们可分为可溶性淀粉合成酶ⅰ（SSI）、可溶性淀粉合成酶ⅰ（SSII）、可溶性淀粉合成酶ⅱ（SSIII）、可溶性淀粉合成酶ⅴ（SSIV）和颗粒结合淀粉合成酶（GBSS） 5类。在水稻中，SSI基因有1个，SSII基因有3个，SSIII、IV和GBSS基因各有2个。采用半定量RT-PCR方法检测了这10个基因在颖果发育过程中的表达规律。根据时间表达模式，10个基因可分为3组：(i)早期表达基因（SSII-2、III-1、GBSSII），在灌浆早期表达；（ii）后期表达子（SSII-3、III-2、GBSSI），在灌浆中后期表达；（iii）稳定表达子（SSI、II-1、IV-1、IV-2），在灌浆过程中表达相对稳定。在颖果内，这三个基因群在空间上共享它们的表达，即。果皮中的“早期表达者”，胚乳中的“晚期表达者”和两个组织中的“稳定表达者”。此外，这种分组也反映在水稻各组织的表达模式上：在非胚乳、胚乳或所有组织中表达。讨论了淀粉合成等基因的时空工作共享的意义。
To elucidate the roles of the isogenes encoding starch synthase (EC 2.4.1.21) in rice (Oryza sativa L.), a comprehensive expression analysis of the gene family was conducted. Extensive searches for starch synthase genes were done in the databases of both the whole genome and full-length cDNAs of rice, and ten genes were revealed to comprise the starch synthase gene family. Multi-sequence alignment analysis of the starch synthase proteins from rice and other plant species suggested that they were grouped into five classes, soluble starch synthase I (SSI), SSII, SSIII, SSIV and granule-bound starch synthase (GBSS). In rice, there was one gene for SSI, three for SSII and two each for SSIII, IV and GBSS. The expression pattern of the ten genes in the developing caryopsis was examined by semi-quantitative RT-PCR analysis. Based on the temporal expression patterns, the ten genes could be divided into three groups: (i) early expressers (SSII-2, III-1, GBSSII), which are expressed in the early stage of grain filling; (ii) late expressers (SSII-3, III-2, GBSSI), which are expressed in the mid to later stage of grain filling; and (iii) steady expressers (SSI, II-1, IV-1, IV-2), which are expressed relatively constantly during grain filling. Within a caryopsis, the three gene groups spatially share their expression, i.e. "early expressers" in the pericarp, the "late expressers" in the endosperm" and the "steady expressers" in both tissues. In addition, this grouping was reflected in the expression pattern of various rice tissues: expression in non-endosperm, endosperm or all tissues examined. The implications in this spatio-temporal work sharing of starch synthesis isogenes are discussed.