Electrospinning of nano/micro scale poly(L-lactic acid) aligned fibers and their potential in neural tissue engineering
Electrospinning of nano/micro scale poly(L-lactic acid) aligned fibers and their potential in neural tissue engineering
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DOI：
10.1016/j.biomaterials.2004.06.051
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发表时间：
2005-05-01
期刊：
影响因子：
14
通讯作者：
Ramakrishna, S
中科院分区：
文献类型：
--
作者：
Yang, F;Murugan, R;Ramakrishna, S
本研究描述了定向聚L-乳酸（PLLA）纳米/微米纤维支架用于神经组织工程的有效性，并与随机PLLA支架的性能进行了比较。采用静电纺丝技术在最佳条件下制备了具有良好取向性的聚乳酸纤维支架，并通过调节聚合物溶液的浓度可以方便地调节纤维的直径。由于PLLA支架的结构旨在用于神经组织工程，因此使用神经干细胞（NSCs）作为模型细胞系体外评价其适用性。通过各种显微技术研究细胞形态、分化和轴突生长。结果表明，在定向支架中，神经干细胞的伸长和突起生长方向与PLLA纤维的方向平行。没有观察到细胞取向相对于纤维直径的显著变化。然而，PLLA纳米纤维的NSC分化率高于微米纤维，并且与纤维排列无关。实验结果表明，聚乳酸纳米纤维定向支架可作为神经组织工程的细胞载体。(C)2004爱思唯尔有限公司保留所有权利。
Efficacy of aligned poly(L-lactic acid) (PLLA) nano/micro fibrous scaffolds for neural tissue engineering is described and their performance with random PLLA scaffolds is compared as well in this study. Perfectly aligned PLLA fibrous scaffolds were fabricated by an electrospinning technique under optimum condition and the diameter of the electrospun fibers can easily be tailored by adjusting the concentration of polymer solution. As the structure of PLLA scaffold was intended for neural tissue engineering, its suitability was evaluated in vitro using neural stem cells (NSCs) as a model cell line. Cell morphology, differentiation and neurite outgrowth were studied by various microscopic techniques. The results show that the direction of NSC elongation and its neurite outgrowth is parallel to the direction of PLLA fibers for aligned scaffolds. No significant changes were observed on the cell orientation with respect to the fiber diameters. However, the rate of NSC differentiation was higher for PLLA nanofibers than that of micro fibers and it was independent of the fiber alignment. Based on the experimental results, the aligned nanofibrous PLLA scaffold could be used as a potential cell carrier in neural tissue engineering. (C) 2004 Elsevier Ltd. All rights reserved.