The primary structure and expression of four cloned human histone genes.
The primary structure and expression of four cloned human histone genes.
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四个克隆的人类组蛋白基因的一级结构和表达。
DOI：
10.1093/nar/11.21.7409
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发表时间：
1983
影响因子：
14.9
通讯作者：
Heintz,N
中科院分区：
文献类型：
--
作者：
Zhong,R;Roeder,RG;Heintz,N
四种人类组蛋白基因的完整核苷酸序列已确定。每个基因编码一个核心组蛋白，该蛋白与相应的小牛胸腺或大鼠历史非常同源。湖南组蛋白基因的5'和31侧翼区域包含先前鉴定的共有序列：TATA盒、GACTTC元件； CCAAT序列； 3' 末端二元组对称元件被认为与转录终止有关；以及最近鉴定的 H2b 特定上游序列。假定的 H2a 特异性上游序列 5'-TTTCTTGGACTCCTCTTTTC-3' 存在于湖南 H2a 基因启动子中 TATA 盒上游约 40 个碱基对处。对每个克隆内编码的人组蛋白 mRNA 进行核酸酶 SI 分析表明，raRNA 末端相对于已知共有序列映射到预期位置，并且每个 mRNA 的丰度在 HeLa 细胞周期期间受到调节。最后，与分别在克隆pHh4A/pHh4C、pHh5B和pHh4A内编码的H2b、H3和H4 mRNA相比，由Hh5G编码的H2a mRSA不存在于人胎盘RNA中。
The complete nucleotide sequence of four human histone genes has been determined. Each gene codes for a core histone protein which is very homologous with the corresponding calf thymus or rat histories. The 5' and 31 flanking regions of the hunan histone genes contain previously identified concensus sequences: the TATA box, the GACTTC element; the CCAAT sequence; the 3' terminal dyad symmetry element thought to be involved in transcription termination; and a recently identified H2b specific upstream sequence. A putative H2a specific upstream sequence 5′-TTCTTGGACTCCTCTTTTC-3′ is present approximately 40 base pairs upstream from the TATA box in the hunan H2a gene promoter. Nuclease SI analysis of the human histone mRNAs encoded within each of these clones demonstrates that the raRNA terminii map to the expected positions relative to the known concensus sequences, and that the abundance of each mRNA is regulated during the HeLa cell cycle. Finally, in contrast to the H2b, H3 and H4 mRNAs encoded within clones pHh4A/pHh4C,pHh5B and pHh4A, respectively, the H2a mRSA encoded by Hh5G is not present in human placental RNA.