Creation of "humanized" mice to study human immunity.
Creation of "humanized" mice to study human immunity.
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DOI：
10.1002/0471142735.im1521s81
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发表时间：
2008-05-01
影响因子：
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通讯作者：
Shultz, Leonard D
中科院分区：
文献类型：
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作者：
Pearson, Todd;Greiner, Dale L;Shultz, Leonard D
“人源化”小鼠是研究人类造血和免疫的一种很有前途的翻译模型。随着新的免疫缺陷宿主种群的开发，它们的实用性得到了增强，最引人注目的是缺乏IL-2受体共同伽马链的NOD-SCID IL2rGamma(空)小鼠等小鼠品系。这些小鼠缺乏适应性免疫功能，在天然免疫方面表现出多种缺陷，并支持高水平的人血淋巴植入。人源化的小鼠可以支持免疫学的许多领域的研究，包括自身免疫、移植、传染病和癌症。这些模型在没有合适的人类疾病小动物模型的实验中特别有价值，例如在某些病毒感染的情况下。本单元详细介绍了通过将免疫缺陷小鼠植入造血干细胞或外周血单核细胞来创建人源化小鼠，提供了评估植入的方法，并讨论了选择适当的模型系统以满足特定目标的考虑因素。
"Humanized" mice are a promising translational model for studying human hematopoiesis and immunity. Their utility has been enhanced by the development of new stocks of immunodeficient hosts, most notably mouse strains such as NOD-scid IL2rgamma(null) mice that lack the IL-2 receptor common gamma chain. These stocks of mice lack adaptive immune function, display multiple defects in innate immunity, and support heightened levels of human hematolymphoid engraftment. Humanized mice can support studies in many areas of immunology, including autoimmunity, transplantation, infectious diseases, and cancer. These models are particularly valuable in experimentation where there is no appropriate small animal model of the human disease, as in the case of certain viral infections. This unit details the creation of humanized mice by engraftment of immunodeficient mice with hematopoietic stem cells or peripheral blood mononuclear cells, provides methods for evaluating engraftment, and discusses considerations for choosing the appropriate model system to meet specific goals.