Effects of Microcystin-LR Exposure on Spermiogenesis in Nematode Caenorhabditis elegans.
Effects of Microcystin-LR Exposure on Spermiogenesis in Nematode Caenorhabditis elegans.
复制标题
微囊藻毒素-LR 暴露对线虫秀丽隐杆线虫精子发生的影响
DOI：
10.3390/ijms160922927
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发表时间：
2015-09-22
影响因子：
5.6
通讯作者：
Pu Y
中科院分区：
文献类型：
--
作者：
Li Y;Zhang M;Chen P;Liu R;Liang G;Yin L;Pu Y
关于微囊藻毒素-亮氨酸精氨酸(MC-LR)对精子发生的影响，人们知之甚少，尽管这些数据对于更好地阐明生殖健康是非常重要的。在本工作中，我们以线虫秀丽线虫(C.elegans)为动物模型，研究MC-LR对精子发生的影响。结果表明，MC-LR暴露可引起精子形态异常，并导致精子激活、反式激活、精子行为和竞争等严重缺陷。低于16.0μg/L的MC-LR可显著降低线虫SPE-15的表达水平，而暴露于1.0μg/L的MC-LR可显著降低线虫SPE-10和FER-1的表达水平。因此，本研究表明MC-LR对精子发生有不良影响，这些精子功能缺陷可能与线虫Spe-10、Spe-15和FER-1基因表达降低有关。
Little is known about the effect on spermiogenesis induced by microcystin-leucine arginine (MC-LR), even though such data are very important to better elucidate reproductive health. In the current work, with the aid of nematode Caenorhabditis elegans (C. elegans) as an animal model, we investigated the defects on spermiogenesis induced by MC-LR. Our results showed that MC-LR exposure induced sperm morphology abnormality and caused severe defects of sperm activation, trans-activation, sperm behavior and competition. Additionally, the expression levels of spe-15 were significantly decreased in C. elegans exposed to MC-LR lower than 16.0 μg/L, while the expression levels of spe-10 and fer-1 could be significantly lowered in C. elegans even exposed to 1.0 μg/L of MC-LR. Therefore, the present study reveals that MC-LR can induce adverse effects on spermiogenesis, and those defects of sperm functions may be induced by the decreases of spe-10, spe-15 and fer-1 gene expressions in C. elegans.