In vivo Visualization of the Distribution of a Secretory Protein in Aspergillus oryzae Hyphae Using the RntA-EGFP Fusion Protein
In vivo Visualization of the Distribution of a Secretory Protein in Aspergillus oryzae Hyphae Using the RntA-EGFP Fusion Protein
复制标题
使用 RntA-EGFP 融合蛋白体内可视化米曲霉菌丝中分泌蛋白的分布
DOI：
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发表时间：
2003
期刊：
影响因子：
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通讯作者：
K. Kitamoto
中科院分区：
文献类型：
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作者：
K. Masai;J. Maruyama;H. Nakajima;K. Kitamoto
构建并表达了编码核糖核酸酶T1-EGFP的融合基因(rNTA-EGFP)，为研究米曲霉的分泌途径奠定了基础。荧光检测和Western分析检测到RNTA-EGFP融合蛋白的成功分泌。利用RntA-EGFP系统，我们能够在最佳条件下观察到生长过程中基本细胞的菌丝顶端和隔膜处的强荧光。生长过程中的冷激或热激均可引起液泡中EGFP荧光的积累。
A fusion gene encoding ribonuclease T1-EGFP (rntA-egfp) was constructed and expressed to use it as a tool for studies on the secretory pathway in Aspergillus oryzae. The successful secretion of the intact RntA-EGFP fusion protein was detected by fluorescence measurement and Western analysis. With use of the RntA-EGFP system, we were able to see high fluorescence at hyphal tips and observe concentrated fluorescence at septa in basal cells during growth at optimal conditions. Cold or heat shock during growth caused the accumulation of EGFP fluorescence in vacuoles.