Glycosaminoglycans and proteoglycans from different zones of the porcine knee meniscus
Glycosaminoglycans and proteoglycans from different zones of the porcine knee meniscus
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DOI：
10.1002/jor.1100150209
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发表时间：
1997-03-01
影响因子：
2.8
通讯作者：
Scott, PG
中科院分区：
文献类型：
--
作者：
Nakano, T;Dodd, CM;Scott, PG
从20周龄的猪中获得内侧和外侧膝关节韧带，解剖成三个相等宽度的区域，并分析胶原和糖胺聚糖含量以及糖胺聚糖和蛋白聚糖的类型。薄的内部区域含有约76%的胶原蛋白和8%的糖胺聚糖（以干重计），外部区域含有93%的胶原蛋白和2%的糖胺聚糖。所有带中最丰富的糖胺聚糖是硫酸软骨素，约占内带总糖胺聚糖的80%，在外带中占50-56%。硫酸皮肤素是第二丰富的糖胺聚糖，相对于硫酸软骨素在内区中以约1：5-6的比例存在，在外区中以1：1.5的比例存在。内区中的透明质酸占总糖胺聚糖含量的4-5%，外区中占10%。中间带的所有成分参数均介于内带和外带之间。内侧和外侧椎间盘的成分无统计学显著差异。提取蛋白聚糖，并通过凝胶色谱法分离成两组（大蛋白聚糖和小蛋白聚糖），并通过凝胶电泳进一步表征。使用硫酸软骨素和硫酸角质素的单克隆抗体对大蛋白聚糖进行染色。双糖链蛋白聚糖和核心蛋白聚糖，两个相关的硫酸皮肤素蛋白聚糖，在小的蛋白聚糖池中确定了他们的行为凝胶电泳和免疫染色的特异性抗体。在中部和内部区域，biglycan占主导地位。观察到较低的电泳迁移率的硫酸皮肤素蛋白聚糖从内部区域相比，从外部区域的解释发现较长的硫酸皮肤素链的前。总的来说，这些结果表明，膝关节半月板的细胞外基质在其宽度上连续变化，其变化方式与结构较薄的内部方面的压缩载荷增加一致。
Medial and lateral knee menisci were obtained from 20-week-old pigs, dissected into three zones of equal width, and analyzed for collagen and glycosaminoglycan content and for types of glycosaminoglycan and proteoglycan. The thin inner zones contained about 76% collagen and 8% glycosaminoglycan (by dry weight) and the outer zones, 93% collagen and 2% glycosaminoglycan. The most abundant glycosaminoglycan in al zones was chondroitin sulphate, accounting for about 80% of total glycosaminoglycan in the inner zones and 50-56% in the outer zones. Dermatan sulphate was the second most abundant glycosaminoglycan, present relative to chondroitin sulphate in a ratio of about 1:5-6 in the inner zones and 1:1.5 in the outer zones. Hyaluronic acid accounted for 4-5% of total glycosaminoglycan content in the inner zones and 10% in the outer zones. All compositional parameters for the middle zones were between those for the inner and outer zones. There were no statistically significant differences in composition between medial and lateral menisci. Proteoglycans were extracted and separated into two groups (large and small proteoglycans) by gel chromatography and were further characterized by gel electrophoresis. The large proteoglycans stained with use of monoclonal antibodies to chondroitin sulphate and keratan sulphate. Biglycan and decorin, two related dermatan sulphate proteoglycans, were identified in the small proteoglycan pool by their behaviour on gel electrophoresis and by immunostaining with specific antibodies. In the middle and inner zones, biglycan predominated. The observed lower electrophoretic mobilities of dermatan sulphate proteoglycans from the inner zone compared with those from the outer zone were explained by the discovery of longer dermatan sulphate chains on the former. Collectively, these results show that the extracellular matrix of knee meniscus varies continuously across its width in a manner consistent with increased compressive loading on the thinner, inside aspect of the structure.