Absence of p53 gene mutations in hepatocarcinomas from a Mediterranean area of Spain -: A study of 129 archival tumour samples
Absence of p53 gene mutations in hepatocarcinomas from a Mediterranean area of Spain -: A study of 129 archival tumour samples
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DOI：
10.1007/s004280050374
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发表时间：
1999-06-01
期刊：
影响因子：
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通讯作者：
Llombart-Bosch, A
中科院分区：
文献类型：
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作者：
Boix-Ferrero, J;Pellín, A;Llombart-Bosch, A
人肝细胞癌（HCC）中p53基因异常的发生率在不同的地理区域不同，在肝炎病毒感染和饮食暴露于黄曲霉毒素BI是最常见病原体的区域更高。这些突变在欧洲不太常见，尽管一些研究报告了高达45%的分析病例中p53蛋白过表达。我们分析了129例原发性恶性肝肿瘤的肿瘤样本，这些样本是从西班牙地中海地区（瓦伦西亚和赫罗纳）的两个病理实验室处理的石蜡块中回收的。14例p53免疫组化染色阳性者，5例阳性细胞核占50%以上。PCR-SSCP分析结果显示，70例患者外显子5 ~ 8全序列，其余患者外显子5 ~ 8部分序列均为缺失，未发现突变，与临床分期、肿瘤分期及临床预后无关。我们的结论是，p53基因的改变是不是一个主要的事件，在地中海的这一地区的肝细胞的恶性转化。在其他地理区域p53基因改变的可变发生率可能反映了HCC病因学的不同遗传背景。
The incidence of p53 gene abnormalities in human hepatocellular carcinoma (HCC) varies in different geographical areas, being higher in regions where hepatitis virus infection and dietary exposure to aflatoxin BI are the most common aetiological agents. These mutations are less frequently encountered in Europe, although some studies have reported p53 protein overexpression in up to 45% of cases analysed. We have analy sed 129 tumour samples of primary malignant hepatic neoplasms recovered from paraffin blocks processed in two pathology laboratories in a Mediterranean area of Spain (Valencia and Gerona). Among 14 cases in which p53 immunohistochemistry expression proved positive, 5 stained in more than 50% of the cell nuclei. By PCR-SSCP analysis we could detect the complete sequence from exon 5 through 8 in 70 cases and parr of this region in the remaining cases, but no mutations were found. We found no relationship with the clinical stage, tumour stage or clinical outcome. We conclude that p53 gene alterations are not a major event in the malignant transformation of hepatic cells in this region of the Mediterranean. The variable incidence of p53 gene alterations in other geographical areas may reflect a different genetic background for the aetiology of HCC.