LEAD-INTOXICATION DURING DEVELOPMENT - ITS LATE EFFECTS ON KIDNEY-FUNCTION AND BLOOD-PRESSURE
LEAD-INTOXICATION DURING DEVELOPMENT - ITS LATE EFFECTS ON KIDNEY-FUNCTION AND BLOOD-PRESSURE
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DOI：
10.1038/ki.1980.51
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发表时间：
1980-01-01
影响因子：
19.6
通讯作者：
SPITZER, A
中科院分区：
文献类型：
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作者：
AVIV, A;JOHN, E;SPITZER, A
方法将3周龄SD大鼠按性别和体重配对，随机分为两组，实验组饮用1%醋酸铅溶液，对照组饮用自来水。铅暴露被限制在3至9周龄的6周内。所有大鼠都被允许自由进食大鼠食物(新泽西州Ralston Purina Co.)。每对大鼠在12周龄或25周龄时进行研究。大鼠用肌动蛋白®(10mgI 100g体质量，ip)麻醉，然后放在恒温控制的床上。聚乙烯导管(PE-50；Clay Adams Co.)经右颈静脉注入利沙明绿，右股动脉采血，右股静脉输液。膀胱通过耻骨上切开露出。放置两根PE-50导管：一根在膀胱，另一根在左输尿管。左肾用先前描述的方法准备进行显微穿刺术[34]。手术准备结束后立即给予等量生理盐水0.5ml100g体重，含25Ci的0.9%生理盐水溶液。然后以0.5mLhr/100g体重的速率注入14C-菊粉(新英格兰核)。经过45 mm的平衡期后，收集2~3次尿液，每次30 mm，并定时采集近端肾小管液。抽取血液样本，在每个周期的中点测量动脉血压。这些样品用于测量GFR和SINGLE
Methods Three-week-old littermates (Sprague-Dawley rats) were paired according to weight and sex, and were randomly assigned to either an experimental group, which received 1% lead acetate as a drinking solu-tion, or to a control group, which received tap water. Lead exposure was limited to the 6-week pe-riod corresponding to 3 to 9 weeks of age. All rats were allowed free acess to rat chow (Ralston Purina Co., New Jersey). Each pair was studied either at 12 or at 25 weeks of age.The rats were anesthetized with mactin®(10 mgI 100 g body wt, ip) and then were placed on a ther-mostatically controlled table. Polyethylene catheters (PE-50; Clay Adams Co.) were inserted into the right jugular vein for injection of lissamine green, the right femoral artery for blood sampling, and the right femoral vein for infusion of fluids. The bladder was exposed through a suprapubic incision. Two PE-50 catheters were positioned: one in the bladder, the other in the left ureter. The left kidney was prepared for micropuncture by a previously de-scribed method [34]. Isotonic saline in a volume equivalent to 0.5 ml! 100 g body wt was given immediately after surgical preparation of the animal, and a 0.9% saline solution containing 25 Ci! ml of 14C-inulin (New England Nuclear) was infused there-after at a rate of 0.5 mL'hr/lOO g body wt. Following a 45-mm equilibration period, two to three urine col-lections, each of 30 mm's duration, were obtained, and timed collections of proximal tubular fluid were performed. Blood samples were withdrawn, and measurements of arterial blood pressure were made at the midpoint of each period. These samples served for the measurements of GFR and single