Involvement of nectins in the formation of puncta adherentia junctions and the mossy fiber trajectory in the mouse hippocampus
Involvement of nectins in the formation of puncta adherentia junctions and the mossy fiber trajectory in the mouse hippocampus
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DOI：
10.1016/j.mcn.2005.10.002
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发表时间：
2006-02-01
影响因子：
3.5
通讯作者：
Takai, Y
中科院分区：
文献类型：
--
作者：
Honda, T;Sakisaka, T;Takai, Y
突触是细胞间的特化连接，其特异性和可塑性由突触细胞粘附分子介导。在海马中，苔藓纤维在CA3锥体细胞的顶端树突上形成突触，其中突触和附着点连接（PAJs）高度发达。突触连接是神经传递的部位，paj被认为是机械粘附的部位。细胞-细胞粘附分子nectin-1和nectin-3分别不对称地定位于paj的突触前和突触后侧。为了揭示连接素的明确作用，我们分析了连接素-1（-/-）和连接素-3（-/-）小鼠。在这两种突变小鼠中，苔藓纤维终端和CA3锥体细胞树突之间的突触上的paj数量减少。此外，还观察到异常的苔藓纤维轨迹。这些结果表明，连接蛋白参与了paj的形成，paj维持了苔藓纤维的正常轨迹。(c) 2005爱思唯尔公司版权所有。
Synapses are specialized intercellular junctions whose specificity and plasticity are mediated by synaptic cell adhesion molecules. In hippocampus, the mossy fibers form synapses on the apical dendrites of the CA3 pyramidal cells where synaptic and puncta adherentia junctions (PAJs) are highly developed. Synaptic junctions are the sites of neurotransmission, while PAJs are regarded as mechanical adhesion sites. Cell-cell adhesion molecules nectin-1 and nectin-3 asymmetrically localize at the pre- and post-synaptic sides of PAJs, respectively. TO reveal the definitive role of nectins, we analyzed nectin-1(-/-) and nectin-3(-/-) mice. In both the mutant mice, the number of PAJs at the synapses between the mossy fiber terminals and the dendrites of the CA3 pyramidal cells was reduced. In addition, the abnormal mossy fiber trajectory was observed. These results indicate that nectins are involved in the formation of PAJs, which maintain the proper mossy fiber trajectory. (c) 2005 Elsevier Inc. All rights reserved.