Characterization of Porphyrobacter sanguineus sp nov., an aerobic bacteriochlorophyll-containing bacterium capable of degrading biphenyl and dibenzofuran
Characterization of Porphyrobacter sanguineus sp nov., an aerobic bacteriochlorophyll-containing bacterium capable of degrading biphenyl and dibenzofuran
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DOI：
10.1007/s00203-002-0423-5
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发表时间：
2002-07-01
影响因子：
2.8
通讯作者：
Kawahara, K
中科院分区：
文献类型：
--
作者：
Hiraishi, A;Yonemitsu, Y;Kawahara, K
从系统发育和分类学的角度对3株血红土壤杆菌（Agrobacterium sanguineum）进行了重新鉴定。16 S rDNA序列比较表明，“A.“血红菌”菌株属于α-变形菌门的α-4亚群，其中红微菌属和卟啉杆菌属的成员是它们的近亲。DNA-DNA杂交研究表明，A.“sanguineum”菌株可与这些属的任何先前已知的物种区分开。在“A”中检测到细菌叶绿素a、单糖型鞘糖脂、C14：0、C15：0、C16：0和C16：1的2-OH脂肪酸和泛醌-10。sanguineum”菌株。DNA的G+C为63.8- 64.0mol%。两个“A。“sanguineum”菌株，IAM 12620（=ATCC 25659）和ATCC 25661，能够在0.05%酵母提取物存在下以联苯和二苯并呋喃作为唯一碳源生长。由于可溶性显色代谢物的释放，这些培养物中的培养基在生长的指数期变成黄橙色。剩下的“A。血红菌”菌株ATCC 25660，以及所有的赤微菌属和卟啉杆菌属的测试菌株既不生长也不产生具有联苯或二苯并呋喃的黄橙色色素。在PCR实验中，检测到了编码联苯双加氧酶大亚基蛋白的bphA 1基因。sanguineum”IAM 12620和ATCC 25661。基于这些结果，我们建议将“A. Sanguineum”IAM 12620和ATCC 25661作为一种新的紫杆菌属物种，命名为Sanguineus Porphyrobacter sp. nov.
Three strains of "Agrobacterium sanguineum", an aerobic marine bacterial species described previously, were re-characterized from phylogenetic and taxonomic viewpoints. 16S rDNA sequence comparisons showed that the "A. sanguineum" strains belong to the alpha-4 subgroup of alpha-Proteobacteria, with members of the genera Erythromicrobium and Porphyrobacter as their closest relatives. DNA-DNA hybridization studies indicated that the "A. sanguineum" strains were distinguishable from any previously known species of these genera. Bacteriochlorophyll a, monosaccharide-type glycosphingolipids, 2-OH fatty acids of C14:0, C15:0, C16:0, and C16:1, and ubiquinone-10 were detected in the "A. sanguineum" strains. The G+C of the DNA was 63.8-64.0 mol%. Two of the "A. sanguineum" strains, IAM 12620 (=ATCC 25659) and ATCC 25661, were able to grow with biphenyl and dibenzofuran as sole carbon source in the presence of 0.05% yeast extract. The medium in these cultures turned yellowish-orange at the exponential phase of growth due to the release of soluble chromogenic metabolites. The remaining "A. sanguineum" strain, ATCC 25660, and all test strains of Erythromicrobium and Porphyrobacter neither grew nor produced yellow-orange pigment with biphenyl or dibenzofuran. In PCR experiments, bphA1 gene, coding for the large subunit protein of biphenyl dioxygenase, was detected in "A. sanguineum" IAM 12620 and ATCC 25661. Based on these results, we propose classifying "A. sanguineum" IAM 12620 and ATCC 25661 as a new species of the genus Porphyrobacter with the name Porphyrobacter sanguineus sp. nov.