Leakage-free bonding of porous membranes into layered microfluidic array systems
Leakage-free bonding of porous membranes into layered microfluidic array systems
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DOI：
10.1021/ac062118p
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发表时间：
2007-05-01
影响因子：
7.4
通讯作者：
Takayama, Shuichi
中科院分区：
文献类型：
--
作者：
Chueh, Bor-han;Huh, Dongeun;Takayama, Shuichi
将半多孔膜集成到聚二甲基硅氧烷（PDMS）微流体装置中可用于质量传递控制。存在几种方法，例如等离子体氧化和PDMS预聚物的手动施加，以将这种膜夹在简单的通道结构中，但是这些方法难以实现可靠的密封并且对于具有复杂通道特征的装置没有泄漏或堵塞。本文描述了一种简单但稳健的策略，以有效地将半多孔聚酯和聚碳酸酯膜粘合在PDMS微通道结构层之间，而不会堵塞通道。将旋涂在载玻片上的PDMS预聚物薄层转移到具有通道特征的PDMS基底上以及通过冲压转移到半多孔膜的边缘上。这种薄的PDMS预聚物用作“砂浆”，以牢固地粘合两个PDMS层并密封由膜的厚度产生的裂缝。这种结合方法使得能够制造具有96种流体相互作用组合的8 X 12交叉微流体通道阵列。通过测量细胞对不同颜色荧光试剂的响应，证明了该装置用于生物分析的能力。
The integration of semiporous membranes into poly(dimethylsiloxane) (PDMS) microfluidic devices is useful for mass transport control. Several methods such as plasma oxidation and manual application of PDMS prepolymer exist to sandwich such membranes into simple channel structures, but these methods are difficult to implement with reliable sealing and no leakage or clogging for devices with intricate channel features. This paper describes a simple but robust strategy to bond semiporous polyester and polycarbonate membranes between layers of PDMS microchannel structures effectively without channel clogging. A thin layer of PDMS prepolymer, spin-coated on a glass slide, is transferred to PDMS substrates with channel features as well as to the edges of the semiporous membrane by stamping. This thin PDMS prepolymer serves as "mortar" to strongly bond the two PDMS layers and seal off the crevices generated from the thickness of the membranes. This bonding method enabled the fabrication of an 8 x 12 criss-crossing microfluidic channel array with 96 combinations of fluid interactions. The capability of this device for bioanalysis was demonstrated by measuring responses of cells to different color fluorescent reagents.