Analysis of mouse oocyte activation suggests the involvement of sperm perinuclear material
Analysis of mouse oocyte activation suggests the involvement of sperm perinuclear material
复制标题
DOI：
10.1095/biolreprod58.6.1407
复制
发表时间：
1998-06-01
影响因子：
3.6
通讯作者：
Yanagimachi, H
中科院分区：
文献类型：
--
作者：
Kimura, Y;Yanagimachi, R;Yanagimachi, H
小鼠的卵母细胞可以通过注射单个完整的小鼠精子或其分离的头部来激活。孤立的尾巴不能激活卵母细胞。活性精子携带卵母细胞激活因子(SOAF)存在于圆形精子细胞向精子转化的过程中。SOAF的作用不是高度物种特异性的：小鼠卵母细胞通过注射外来物种的精子来激活，如仓鼠、兔子、猪、人，甚至鱼。通过对(仓鼠)精子的简单冻融，可以提取一部分SOAF；用Triton X-100和十二烷基硫酸钠连续处理精子可以获得额外的SOAF。在SOAF提取过程中对精子头部的电子显微镜检查表明，相对不溶的SOAF与核周物质有关。当通过显微手术将Triton X-100处理的精子头注射到卵母细胞中时，可以激活卵母细胞，导致正常的胚胎发育。虽然核周成分被认为是纯粹的结构性作用，但这些数据表明，它们在激活卵母细胞方面具有额外的功能。
The mouse oocyte can be activated by injection of a single, intact mouse spermatozoon or its isolated head. Isolated tails are unable to activate the oocyte. Active sperm-borne oocyte-activating factor(s) (SOAF) appears during transformation of the round spermatid into the spermatozoon. The action of SOAF is not highly species-specific: mouse oocytes are activated by injection of spermatozoa from foreign species, such as the hamster, rabbit, pig, human, and even fish. Some SOAF can be extracted by simple freeze-thawing of (hamster) spermatozoa; additional SOAF is obtained by sequential treatment of spermatozoa with Triton X-100 and SDS. Electron microscopic examination of sperm heads during SOAF extraction suggests that the relatively insoluble SOAF is associated with perinuclear material. When microsurgically injected into oocytes, Triton X-100-treated sperm heads (with perinuclear material, but without any membranes) can activate the oocytes, leading to normal embryonic development. Whereas perinuclear components have been believed to play a purely structural role, these data suggest an additional function for them in oocyte activation.