METABOLIC ANATOMY OF BRAIN - A COMPARISON OF REGIONAL CAPILLARY DENSITY, GLUCOSE-METABOLISM, AND ENZYME-ACTIVITIES
METABOLIC ANATOMY OF BRAIN - A COMPARISON OF REGIONAL CAPILLARY DENSITY, GLUCOSE-METABOLISM, AND ENZYME-ACTIVITIES
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DOI：
10.1002/cne.902880304
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发表时间：
1989-10-15
影响因子：
2.5
通讯作者：
COLLINS, RC
中科院分区：
文献类型：
--
作者：
BOROWSKY, IW;COLLINS, RC
比较了大鼠脑毛细血管密度、葡萄糖利用率、糖酵解酶乳酸脱氢酶和线粒体酶细胞色素氧化酶的活性的区域变化。用组织化学染色技术研究毛细血管和酶的分布，用[14 C]2-脱氧葡萄糖放射自显影法测定葡萄糖代谢。对18个灰质区和5个白色区的分析显示毛细血管密度与葡萄糖利用率呈正相关。灰质结构中毛细血管密度与乳酸脱氢酶呈负相关。毛细血管和酶的分析也进行分层组织学领域内：海马，嗅球，嗅皮质。一般来说，这揭示了乳酸脱氢酶和细胞色素氧化酶染色的相互模式。毛细血管密度与细胞色素氧化酶活性成正比。乳酸脱氢酶和细胞色素氧化酶的强染色区对应于不同输入途径的突触终末野。这些研究结果表明，糖酵解和氧化酶在脑内的不同分布至少部分与途径特异性相关。
Regional variations in capillary density, glucose utilization rate, and activities of the glycolytic enzyme lactate dehydrogenase and the mitochondrial enzyme cytochrome oxidase were compared in the rat brain. The distributions of capillaries and enzymes were studied by means of histochemical staining techniques, and glucose metabolism was measured by means of [14C]2-deoxyglucose autoradiography. Analysis of 18 gray and five white matter regions revealed a positive correlation between capillary density and glucose utilization rate. A negative correlation was found between capillary density and lactate dehydrogenase among gray matter structures. Analysis of capillaries and enzymes was also performed within laminated histological fields: hippocampus, olfactory bulb, and olfactory cortex. In general, this revealed reciprocal patterns of staining for lactate dehydrogenase and cytochrome oxydase. Capillary density paralleled cytochrome oxidase activity. The zones of intense staining for lactate dehydrogenase and cytochrome oxidase corresponded to the synaptic terminal fields of different input pathways. These findings demonstrate distinct distributions of a glycolytic and an oxidative enzyme within the brain which are at least partly associated with pathway specificity.