Actual Proliferating Index and p53 protein expression as prognostic marker in odontogenic cysts
Actual Proliferating Index and p53 protein expression as prognostic marker in odontogenic cysts
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DOI：
10.1111/j.1601-0825.2009.01590.x
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发表时间：
2009-10-01
期刊：
影响因子：
3.8
通讯作者：
Gawande, M.
中科院分区：
文献类型：
--
作者：
Gadbail, A. R.;Chaudhary, M.;Gawande, M.
背景：本研究的目的是通过观察上皮的实际增殖活性和p53蛋白表达来评估牙源性角化囊肿/角化囊性牙源性肿瘤（KCOT）、根状囊肿（RC）和含牙囊肿（DC）的生物侵袭性。方法：通过Ki-67标记指数和嗜银核仁组织区（AgNOR）计数来测量实际增殖活性每个核。结果：KCOT基底上细胞层中Ki-67阳性细胞较多，分布均匀，RC和DC中少数以基底细胞层为主。 KCOT 基底上细胞层的 AgNOR 计数显着较高。注意到 KCOT 基底上细胞层的实际增殖活性较高。 KCOT 中的基底细胞层和基底上细胞层中 p53 免疫标记密集且分散。弱染色的p53阳性细胞在KCOT中呈弥漫性分布，而它们主要见于RC和DC的基底细胞层。结论：零星KCOT中增殖活性和p53蛋白表达的定量和定性差异可能与内在生长潜力有关，内在生长潜力可能在其发育中发挥作用，并解释局部侵袭性生物学行为。牙源性病变中的 AgNOR 计数和 p53 蛋白检测对于预测生物学行为和预后具有重要意义。
Background:The purpose of this study was to evaluate the biological aggressiveness of odontogenic keratocyst/keratocystic odontogenic tumour (KCOT), radicular cyst (RC) and dentigerous cyst (DC) by observing the actual proliferative activity of epithelium, and p53 protein expression.Methods:The actual proliferative activity was measured by Ki-67 Labelling Index and argyrophilic nucleolar organizing regions (AgNOR) count per nucleus. The p53 protein expression was also evaluated.Results:Ki-67 positive cells were observed higher in suprabasal cell layers of KCOT with uniform distribution, a few of them were predominantly observed in basal cell layer in RC and DC. The AgNOR count was significantly higher in suprabasal cell layers of KCOT. The actual proliferative activity was noted to be higher in suprabasal cell layers of KCOT. The p53 immunolabelling was dense and scattered in basal and suprabasal cell layers in KCOT. The weakly stained p53 positive cells were observed diffusely distributed in KCOT, whereas they were mainly seen in basal cell layer of RC and DC.Conclusion:The quantitative and qualitative differences of the proliferative activity and the p53 protein expression in sporadic KCOT may be associated with intrinsic growth potential that could play a role in its development and explain locally aggressive biological behaviour. AgNOR count and p53 protein detection in odontogenic lesions can be of great consequence to predict the biological behaviour and prognosis.