Muscarinic acetylcholine receptor subtype expression in type vestibular hair cells of guinea pigs
Muscarinic acetylcholine receptor subtype expression in type vestibular hair cells of guinea pigs
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DOI：
10.1007/s11596-011-0582-1
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发表时间：
2011-10
期刊：
影响因子：
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通讯作者：
Qi Yao;Huamao Cheng;Chang-kai Guo;Tao Zhou;Xiang Huang;Weijia Kong
中科院分区：
文献类型：
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作者：
Qi Yao;Huamao Cheng;Chang-kai Guo;Tao Zhou;Xiang Huang;Weijia Kong
最近的研究表明，5个亚型（M1-M5）的毒蕈碱乙酰胆碱受体（mAChR）的前庭周围表达。然而，mAChR的确切细胞位置尚不清楚。本研究采用单细胞RT-PCR技术检测豚鼠前庭II型毛细胞中M1-M5受体mRNA的表达。在前庭终器中，通过RT-PCR获得预期大小的cDNA。此外，mRNA通过RT-PCR从单独分离的II型前庭毛细胞（单细胞RT-PCR）中鉴定。序列分析证实其为M1-M5 mAChR。这些结果表明，M1-M5 mAChR在豚鼠II型前庭毛细胞中表达，这进一步支持了M1-M5 mAChR作为前庭毛细胞传出胆碱能信号通路的介导剂的作用。
Recent studies have demonstrated that five subtypes (M1-M5) of muscarinic acetylcholine receptor (mAChR) are expressed in the vestibular periphery. However, the exact cellular location of the mAChRs is not clear. In this study, we investigated whether there is the expression of M1-M5 muscarinic receptor mRNA in isolated type II vestibular hair cells of guinea pig by using single-cell RT-PCR. In vestibular end-organ, cDNA of the expected size was obtained by RT-PCR. Moreover, mRNA was identified by RT-PCR from individually isolated type II vestibular hair cells (single-cell RT-PCR). Sequence analysis confirmed that the products were M1-M5 mAChR. These results demonstrated that M1-M5 mAChR was expressed in the type II vestibular hair cells of the guinea pig, which lends further support for the role of M1-M5 mAChR as a mediator of efferent cholinergic signalling pathway in vestibular hair cells.