Variation in cytostatic constituents of a sponge-derived Gymnascella dankaliensis by manipulating the carbon source
Variation in cytostatic constituents of a sponge-derived Gymnascella dankaliensis by manipulating the carbon source
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DOI：
10.1021/np070165m
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发表时间：
2007-11-01
影响因子：
5.1
通讯作者：
Numata, Atsushi
中科院分区：
文献类型：
--
作者：
Amagata, Taro;Tanaka, Makoto;Numata, Atsushi
在两种不同的培养基条件下培养软海绵海绵衍生真菌，丹卡利裸盖藻。一种含有可溶性淀粉而不是葡萄糖的改良麦芽提取物培养基产生了两种极不寻常的类固醇，丹卡斯酮A（2）和B（3），而另外四种不寻常的类固醇，裸甾酮A（4）、B（5）、C（6）和D（7）从原始麦芽提取物培养基中分离出来。它们的立体结构已建立在光谱分析的基础上，沿着X-射线晶体结构分析，改进的Mosher方法，CD激子方法，和化学转化。除4种外，所有类固醇均对鼠P388癌细胞系表现出显著的生长抑制作用。丹卡斯特酮A（2）对人癌细胞系也表现出有效的生长抑制作用。
The Halichondria sponge-derived fungus, Gymnacella dankaliensis, was cultured in two different media conditions. A modified malt extract medium containing soluble starch instead of glucose resulted in two extremely unusual steroids, dankasterones A (2) and B (3), while four additional unusual steroids, gymnasterones A (4), B (5), C (6), and D (7), were isolated from the original malt extract medium. Their stereostructures have been established on the basis of spectroscopic analyses along with X-ray crystal structure analyses, modified Mosher's method, CD exciton method, and a chemical transformation. All the steroids except for 4 exhibited significant growth inhibition against the murine P388 cancer cell line. Dankasterone A (2) also exhibited potent growth inhibition against human cancer cell lines.