Expression of microRNA-like RNA-2 (Fgmil-2) and bioH1 from a single transcript in Fusarium graminearum are inversely correlated to regulate biotin synthesis during vegetative growth and host infection
Expression of microRNA-like RNA-2 (Fgmil-2) and bioH1 from a single transcript in Fusarium graminearum are inversely correlated to regulate biotin synthesis during vegetative growth and host infection
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DOI：
10.1111/mpp.12859
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发表时间：
2019-08-06
影响因子：
4.9
通讯作者：
Liao, Yu-Cai
中科院分区：
文献类型：
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作者：
Guo, Mao-Wei;Yang, Peng;Liao, Yu-Cai
MicroRNA-like RNAs （milRNAs）通过转录后下调靶基因。研究了小麦镰刀菌（Fusarium graminearum, Fg）在真菌营养生长和侵染过程中的milRNA表达。小RNA测序鉴定出来自Fg的36个milrna，其中Fgmil-2在分生孢子、菌丝体和感染小麦中每百万有bb100个转录本，在分生孢子中表达量最高，在定植小麦组织中表达量最低。Fgmil-2与参与生物素合成的FgbioH1信使RNA的3MODIFIER LETTER prime - untranslation区域（3MODIFIER LETTER PRIME-UTR）具有完全的同源性。Poly(A)聚合酶介导的cDNA末端快速扩增结合测序分析表明，FgDicer2在FgbioH1转录本的3MODIFIER LETTER PRIME-UTR中的特定位点切割产生具有典型发夹结构的Fgmil-2前体。FgbioH1或FgDicer2基因的缺失会破坏Fgmil-2的生物发生。FgbioH1与Fgmil-2和FgDicer2的表达呈负相关。FgbioH1的缺失也表明它是菌丝生长、毒力、霉菌毒素生物合成和生物素依赖性羧化酶基因表达所必需的。这项研究揭示了Fgmil-2通过其自身的靶转录物FgbioH调控生物素合成的一种新的负相关转录后调控模式。
MicroRNA-like RNAs (milRNAs) post-transcriptionally down-regulate target genes. We investigated Fusarium graminearum (Fg) milRNA expression during fungal vegetative growth and infection of wheat. Small RNA sequencing identified 36 milRNAs from Fg, one of which, Fgmil-2, had >100 transcripts per million in conidia, mycelia and infected wheat, with the highest expression in conidia and the lowest expression in colonized wheat tissue. Fgmil-2 displays perfect homology to the 3MODIFIER LETTER PRIME-untranslated region (3MODIFIER LETTER PRIME-UTR) of an FgbioH1 messenger RNA that is involved in biotin biosynthesis. Poly(A) polymerase-mediated rapid amplification of cDNA ends combined with sequencing analysis demonstrated that cleavage at a specific site by FgDicer2 in the 3MODIFIER LETTER PRIME-UTR of FgbioH1 transcripts generated the Fgmil-2 precursor with a typical hairpin structure. Deletion of FgbioH1 or FgDicer2 genes abolished Fgmil-2 biogenesis. FgbioH1 had an inversely correlated pattern of expression to that of Fgmil-2 and FgDicer2. Deletion of FgbioH1 also showed that it is required for mycelial growth, virulence, mycotoxin biosynthesis and expression of biotin-dependent carboxylase genes. This study reveals in Fg a novel mode of inversely correlated post-transcriptional regulation in which Fgmil-2 originates from its own target transcript, FgbioH, to govern biotin biosynthesis.