Effects of exogenous jasmonic acid on concentrations of direct-defense chemicals and expression of related genes in Bt (Bacillus thuringiensis) corn (Zea mays).
Effects of exogenous jasmonic acid on concentrations of direct-defense chemicals and expression of related genes in Bt (Bacillus thuringiensis) corn (Zea mays).
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DOI：
10.1016/s1671-2927(08)60008-5
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发表时间：
2007-01-01
影响因子：
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通讯作者：
Luo, S. M.
中科院分区：
文献类型：
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作者：
Feng YuanJiao, Feng YuanJiao;Wang JianWu, Wang JianWu;Luo, S. M.
Bt玉米是世界上三大大规模商业化的转基因作物之一。人们越来越清楚地认识到，将作物的内源防御与引入的外源基因相结合的害虫防治的补充持久方法是害虫抗性管理和下一代抗虫转基因作物的有希望的替代策略。采用化学分析、ELISA和RT-PCR等方法，研究了外源茉莉酸（JA）对Bt玉米品种34 B24和非Bt玉米品种34 B23叶片中直接防御化学物质含量、Bt蛋白含量及相关基因表达的诱导效应。结果表明，在34 B24和34 B23中，外源JA均促进了处理叶（第一叶）中LOX、PR-2 α、MPI和PR-1基因的表达。与对照相比，34 B24和34 B23处理的叶片中丁布的浓度分别显著增加63%和18%。34 B24和34 B23的总酚酸也增加了24%和12%。34 B24处理后叶片中Bt蛋白含量增加13%，但第二叶中Bt蛋白含量显著下降27%。34 B24的诱导反应是以系统方式进行的，并且比34 B23强得多。这些结果表明，外源JA诱导的Bt玉米内诱导化学防御系统与Bt基因之间存在协同作用。
Bt corn is one of the top three large-scale commercialized transgenic crops around the world. It is increasingly clear that the complementary durable approaches for pest control, which combine the endogenous defense of the crop with the introduced foreign genes, are promising alternative strategies for pest resistance management and the next generation of insect-resistant transgenic crops. In the present study, we tested the inducible effects of exogenous jasmonic acid (JA) on direct-defense chemical content, Bt protein concentration, and related gene expression in the leaves of Bt corn cultivar 34B24 and non-Bt cultivar 34B23 by chemical analysis, ELISA, and RT-PCR. The results show that the expression of LOX, PR-2alpha, MPI, and PR-1 genes in the treated leaf (the first leaf) was promoted by exogenous JA both in 34B24 and 34B23. As compared with the control, the concentration of DIMBOA in the treated leaf was significantly increased by 63 and 18% for 34B24 and 34B23, respectively. The total phenolic acid was also increased by 24 and 12% for both 34B24 and 34B23. The Bt protein content of 34B24 in the treated leaf was increased by 13% but decreased significantly by 27% in the second leaf. The induced response of 34B24 was in a systemic way and was much stronger than that of 34B23. Those findings indicated that there is a synergistic interaction between Bt gene and internally induced chemical defense system triggered by externally applied JA in Bt corn.